Tumor necrosis factor and immune interferon synergistically increase transcription of HLA class I heavy- and light-chain genes in vascular endothelium

Journal Article · Sun Jul 01 00:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.13.5183· OSTI ID:6249880

Johnson, D R; Pober, J S ^[1]

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1. Harvard Medical School, Boston, MA (USA)

Tumor necrosis factor and immune interferon synergistically increase cell-surface expression of class I major histocompatibility complex molecules in cultured human endothelial cells. The authors report that tumor necrosis factor and interferon {gamma} each independently increase mRNA levels and together cause a greater-than-additive (i.e., synergistic) increase in steady-state mRNA levels and transcriptional rates of the class I heavy- and light-chain genes. HLA heavy-chain mRNA is equally stable in cytokine-treated and -untreated endothelial cells. Interferon {gamma} does not increase tumor necrosis factor receptor number or affinity on human endothelial cells. They conclude that the synergistic increase in class I major histocompatibility complex cell-surface expression results principally from the synergistic increase in transcriptional rates. They propose that this increase is caused by the cooperative binding of independently activated transcription factors to the promoter/enhancer sequences of class I genes.

• OSTI ID: 6249880
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 87:13; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HISTOCOMPATIBILITY COMPLEX
TRANSCRIPTION
INTERFERON
BIOLOGICAL EFFECTS
LYMPHOKINES
CELL CULTURES
ENDOTHELIUM
MESSENGER-RNA
NECROSIS
ANIMAL TISSUES
ANTIGENS
BODY
GROWTH FACTORS
MITOGENS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PROTEINS
RNA
TISSUES
550201* - Biochemistry- Tracer Techniques