Adsorption of cellulase on cellulose: effect of physicochemical properties of cellulose on adsorption and rate of hydrolysis

Journal Article · Mon Nov 01 00:00:00 EST 1982 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260241003· OSTI ID:6247383

Lee, S B; Shin, H S; Ryu, D D.Y.

In the cellulase-cellulose reaction system, the adsorption of cellulase on the solid cellulose substrate was found to be one of the important parameters that govern the enzymatic hydrolysis rate of cellulose. The adsorption of cellulase usually parallels the rate of hydrolysis of cellulose. The affinity for cellulase varies depending on the structural properties of cellulose. Adsorption parameters such as the half-saturation constant, the maximum adsorption constant, and the distribution coefficient for both the cellulase and cellulose have been experimentally determined for several substrates. These adsorption parameters vary with the source of cellulose and the pretreatment methods and are correlated with the crystallinity and the specific surface area of cellulose substrates. The changing pattern of adsorption profile of cellulase during the hydrolysis reaction has also been elucidated. For practical utilization of cellulosic materials, the cellulose structural properties and their effects on cellulase adsorption, and the rate of hydrolysis must be taken into consideration. (Refs. 24).

• Research Organization: The Korea Advanced Inst of Science, PO Box 150, Chong Ryarg Ri Seoul, Korea; US Army Research and Development Lab, Natick, MA 01760
• OSTI ID: 6247383
• Journal Information: Biotechnol. Bioeng.; (United States), Vol. 24:10
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
CELLULASE
ADSORPTION
CELLULOSE
CHEMICAL PROPERTIES
ENZYMATIC HYDROLYSIS
PHYSICAL PROPERTIES
SUBSTRATES
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
HYDROLYSIS
LYSIS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
SORPTION
140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)