Increased leucocyte Na-K ATPase in obesity: reversal following weight loss

Journal Article · Tue Sep 01 00:00:00 EDT 1987 · Metab., Clin. Exp.; (United States)

DOI:https://doi.org/10.1016/0026-0495(87)90093-X· OSTI ID:6245992

Turaihi, K; Baron, D N; Dandona, P

Ouabain-sensitive /sup 86/Rb influx and (/sup 3/H) ouabain binding capacity were investigated in the leucocytes of 17 obese patients and 15 control subjects. Both were significantly increased in the obese when compared with controls. Following dietary restriction and a 4% to 5% weight reduction in the obese over 2 weeks, (/sup 3/H) ouabain binding and ouabain-sensitive /sup 86/Rb influx (a model for K+ influx) decreased to levels similar to those in controls. This shows that the number of Na-K ATPase sites on leucocyte membranes of the obese are significantly increased and that this is associated with accelerated /sup 86/Rb transport. Since both of these indices decreased following 4% to 5% reduction in body weight while the patients were still obese, increased Na-K ATPase is neither a marker of nor cardinal to the pathogenesis of obesity. We conclude that (1) increase in Na-K ATPase units and /sup 86/Rb influx are not characteristic of obesity itself and (2) dietary restriction over the short-term with limited weight reduction restores Na-K ATPase units and /sup 86/Rb influx to normal.

• Research Organization: Royal Free Hospital and School of Medicine, London, England
• OSTI ID: 6245992
• Journal Information: Metab., Clin. Exp.; (United States), Vol. 36:9
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ATP-ASE
RECEPTORS
OUABAIN
BIOCHEMICAL REACTION KINETICS
RUBIDIUM
MEMBRANE TRANSPORT
ADIPOSE TISSUE
CELL MEMBRANES
LEUKOCYTES
PATIENTS
RUBIDIUM 86
TRACER TECHNIQUES
TRITIUM COMPOUNDS
WEIGHT
ACID ANHYDRASES
ALKALI METAL ISOTOPES
ALKALI METALS
ANIMAL TISSUES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CONNECTIVE TISSUE
DAYS LIVING RADIOISOTOPES
DRUGS
ELEMENTS
ENZYMES
GLYCOSIDES
HYDROLASES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
METALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHOHYDROLASES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RUBIDIUM ISOTOPES
STROPHANTHINS
TISSUES
550201* - Biochemistry- Tracer Techniques