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Title: Identification of a cDNA encoding a parathyroid hormone-like peptide from a human tumor associated with humoral hypercalcemia of malignancy

Abstract

Humoral hypercalcemia of malignancy is a common paraneoplastic syndrome that appears to be mediated in many instances by a parathyroid hormone-like peptide. Poly(A)/sup +/ RNA from a human renal carcinoma associated with this syndrome was enriched by preparative electrophoresis and used to construct an enriched cDNA library in phage lambdagt10. The library was screened with a codon-preference oligonucleotide synthesized on the basis of a partial N-terminal amino acid sequence from a human tumor-derived peptide, and a 2.0 kilo-base cDNA was identified. The cDNA encodes a 177 amino acid protein consisting of a 36 amino acid leader sequence and a 141 amino acid mature peptide. The first 13 amino acids of the deduced sequence of the mature peptide display strong homology to human PTH, with complete divergence thereafter. RNA blot-hybridization analysis revealed multiple transcripts in mRNA from tumors associated with the humor syndrome and also in mRNA from normal human keratinocytes. Southern blot analysis of genomic DNA from humans and rodents revealed a simple pattern compatible with a single-copy gene. The gene has been mapped to chromosome 12.

Authors:
; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Yale Univ., New Have, CT (USA)
OSTI Identifier:
6244804
Resource Type:
Journal Article
Journal Name:
Proc. Natl. Acad. Sci. U.S.A.; (United States)
Additional Journal Information:
Journal Volume: 85:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; HETEROCHROMOSOMES; AUTORADIOGRAPHY; MESSENGER-RNA; HYBRIDIZATION; PARATHORMONE; AMINO ACID SEQUENCE; RECOMBINANT DNA; DNA SEQUENCING; CARCINOMAS; ELECTROPHORESIS; GENETIC MAPPING; KIDNEYS; MAN; PEPTIDES; PHOSPHORUS 32; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CHROMOSOMES; DAYS LIVING RADIOISOTOPES; DISEASES; DNA; HORMONES; ISOTOPES; LIGHT NUCLEI; MAMMALS; MAPPING; MOLECULAR STRUCTURE; NEOPLASMS; NUCLEI; NUCLEIC ACIDS; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; PEPTIDE HORMONES; PHOSPHORUS ISOTOPES; PRIMATES; PROTEINS; RADIOISOTOPES; RNA; STRUCTURAL CHEMICAL ANALYSIS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Mangin, M, Webb, A C, Dreyer, B E, Posillico, J T, Ikeda, K, Weir, E C, Stewart, A F, Bander, N H, Milstone, L, and Barton, D E. Identification of a cDNA encoding a parathyroid hormone-like peptide from a human tumor associated with humoral hypercalcemia of malignancy. United States: N. p., 1988. Web. doi:10.1073/pnas.85.2.597.
Mangin, M, Webb, A C, Dreyer, B E, Posillico, J T, Ikeda, K, Weir, E C, Stewart, A F, Bander, N H, Milstone, L, & Barton, D E. Identification of a cDNA encoding a parathyroid hormone-like peptide from a human tumor associated with humoral hypercalcemia of malignancy. United States. https://doi.org/10.1073/pnas.85.2.597
Mangin, M, Webb, A C, Dreyer, B E, Posillico, J T, Ikeda, K, Weir, E C, Stewart, A F, Bander, N H, Milstone, L, and Barton, D E. 1988. "Identification of a cDNA encoding a parathyroid hormone-like peptide from a human tumor associated with humoral hypercalcemia of malignancy". United States. https://doi.org/10.1073/pnas.85.2.597.
@article{osti_6244804,
title = {Identification of a cDNA encoding a parathyroid hormone-like peptide from a human tumor associated with humoral hypercalcemia of malignancy},
author = {Mangin, M and Webb, A C and Dreyer, B E and Posillico, J T and Ikeda, K and Weir, E C and Stewart, A F and Bander, N H and Milstone, L and Barton, D E},
abstractNote = {Humoral hypercalcemia of malignancy is a common paraneoplastic syndrome that appears to be mediated in many instances by a parathyroid hormone-like peptide. Poly(A)/sup +/ RNA from a human renal carcinoma associated with this syndrome was enriched by preparative electrophoresis and used to construct an enriched cDNA library in phage lambdagt10. The library was screened with a codon-preference oligonucleotide synthesized on the basis of a partial N-terminal amino acid sequence from a human tumor-derived peptide, and a 2.0 kilo-base cDNA was identified. The cDNA encodes a 177 amino acid protein consisting of a 36 amino acid leader sequence and a 141 amino acid mature peptide. The first 13 amino acids of the deduced sequence of the mature peptide display strong homology to human PTH, with complete divergence thereafter. RNA blot-hybridization analysis revealed multiple transcripts in mRNA from tumors associated with the humor syndrome and also in mRNA from normal human keratinocytes. Southern blot analysis of genomic DNA from humans and rodents revealed a simple pattern compatible with a single-copy gene. The gene has been mapped to chromosome 12.},
doi = {10.1073/pnas.85.2.597},
url = {https://www.osti.gov/biblio/6244804}, journal = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number = ,
volume = 85:2,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1988},
month = {Fri Jan 01 00:00:00 EST 1988}
}