Insulin regulation of protein biosynthesis in differentiated 3T3 adipocytes. Regulation of glyceraldehyde-3-phosphate dehydrogenase

Journal Article · Sat Oct 05 00:00:00 EDT 1985 · J. Biol. Chem.; (United States)

OSTI ID:6235749

Alexander, M; Curtis, G; Avruch, J; Goodman, H M

The effect of insulin on protein biosynthesis was examined in differentiated 3T3-L1 and 3T3-F442A adipocytes. Insulin altered the relative rate of synthesis of specific proteins independent of its ability to hasten conversion of the fibroblast (preadipocyte) phenotype to the adipocyte phenotype. Although more than one pattern of response to insulin was observed, the authors focused on the induction of a Mr 33,000 protein which was identified as the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Exposure of 3T3 adipocytes to insulin throughout differentiation specifically increased GAPDH activity and protein content by 2- to 3-fold as compared to 3T3 adipocytes differentiated in the absence of insulin. These changes in enzyme activity and content could be accounted for by a 4-fold increase in the relative rate of synthesis of GAPDH and a 9-fold increase in hybridizable mRNA levels. Within 2 h of insulin addition to 3T3 adipocytes differentiated in the absence of hormone, hybridizable GAPDH mRNA levels increased 3-fold, and within 24 h GAPDH mRNA levels increased 8-fold, and (TVS) methionine incorporation into GAPDH protein increased 5-fold. These studies demonstrate that insulin, as the sole hormonal perturbant, can increase the synthesis of certain 3T3 adipocyte proteins by altering the cellular content of a specific mRNA.

• Research Organization: Massachusetts General Hospital, Boston
• OSTI ID: 6235749
• Journal Information: J. Biol. Chem.; (United States), Vol. 22
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HEMIACETAL DEHYDROGENASES
BIOCHEMISTRY
INSULIN
BIOLOGICAL EFFECTS
PROTEINS
BIOSYNTHESIS
ADIPOSE TISSUE
ANIMAL CELLS
CELL DIFFERENTIATION
KINETICS
MESSENGER-RNA
METHIONINE
MICE
MOLECULAR WEIGHT
SULFUR 35
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CHEMISTRY
CONNECTIVE TISSUE
DAYS LIVING RADIOISOTOPES
DRUGS
ENZYMES
EVEN-ODD NUCLEI
HORMONES
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
NUCLEI
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDOREDUCTASES
PEPTIDE HORMONES
RADIOISOTOPES
RNA
RODENTS
SULFUR ISOTOPES
SYNTHESIS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques