Tritium labeled amino acid conjugates of prostaglandins and thromboxanes as labeled ligands in prostanoid radioimmunoassay
Conjugates of prostaglandins and thromboxanes with tritium labeled amino acids were prepared and employed as labeled ligands in prostaglandin and thromboxane radioimmunoassays. Assays for PGF2 alpha, 15-keto-13, 14-dihydro-PGF2 alpha, TXB2 and 15-keto-13,14-dihydro-TXB2 were evaluated in comparative studies using either these heterologous ligands or the corresponding homologous tritiated eicosanoid as tracers. Binding properties for the respective antibodies were found to be similar using either tracer. Three biological studies were also conducted, viz. study of the release of TXB2 during collagen induced platelet aggregation, of 15-keto-13,14-dihydro-TXB2 during guinea pig pulmonary anaphylaxis, and of PGF2 alpha during bovine luteolysis. The analyses gave comparable results using either the heterologous or the homologous assay. Thus, this type of labeled prostanoid conjugates may serve as a convenient alternative to homologous tracers in radioimmunoassay. Heterologous tracers may even in certain cases provide the only simple solution to the problem of preparing a labeled ligand of high specific activity.
- Research Organization:
- Karolinska Institutet, Stockholm, Sweden
- OSTI ID:
- 6234352
- Journal Information:
- Prostaglandins; (United States), Vol. 3
- Country of Publication:
- United States
- Language:
- English
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PROSTAGLANDINS
RADIOIMMUNOASSAY
TRITIUM COMPOUNDS
AMINO ACIDS
LIGANDS
LIQUID COLUMN CHROMATOGRAPHY
CARBOXYLIC ACIDS
CHROMATOGRAPHY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
RADIOASSAY
RADIOIMMUNOLOGY
SEPARATION PROCESSES
TRACER TECHNIQUES
550601* - Medicine- Unsealed Radionuclides in Diagnostics