Effects of thrombin on the integrity of monolayers of cultured human endothelial cells

Journal Article · Wed Sep 01 00:00:00 EDT 1982 · Thromb. Res.; (United States)

DOI:https://doi.org/10.1016/0049-3848(82)90304-8· OSTI ID:6225659

Galdal, K S; Evensen, S A; Brosstad, F

/sup 51/Cr-prelabelled endothelial cells (EC) in confluent monolayers were incubated in RPMI 1640 + foetal calf serum 20% (v/v) to which purified thrombin was added. Thrombin (greater than or equal to 0.1 NIH U/ml) significantly accelerated /sup 51/Cr-release and caused extensive but reversible cell contraction. Thrombin-exposed EC reacted to a new dose of thrombin with no appreciable shape change, but /sup 51/Cr-efflux was again accelerated. EC exposed to thrombin pretreated with N-bromosuccinimide (modifying the macromolecular site) or phenylmethylsulfonyl fluoride (blocking the serine site) retained normal morphology and did not leak excess amounts of /sup 51/Cr. Antithrombin III also inhibited the effect of thrombin. Pretreatment of EC with either indomethacin, aspirin, sulfinpyrazone, pronase or neuraminidase did not influence the effect of subsequent thrombin exposure.

• Research Organization: Research Institute for Internal Medicine, University of Oslo, Norway
• OSTI ID: 6225659
• Journal Information: Thromb. Res.; (United States), Vol. 27:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL CULTURES
MORPHOLOGY
CHROMIUM 51
TRACER TECHNIQUES
THROMBIN
BIOLOGICAL EFFECTS
ENDOTHELIUM
INHIBITION
USA
ANIMAL TISSUES
BETA DECAY RADIOISOTOPES
BODY
CHROMIUM ISOTOPES
COAGULANTS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
NORTH AMERICA
NUCLEI
PEPTIDE HYDROLASES
RADIOISOTOPES
SERINE PROTEINASES
TISSUES
550201* - Biochemistry- Tracer Techniques
550801 - Morphology- Tracer Techniques