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Title: Development of luminescent bacteria as tracers for geological reservoir characterization

Bioluminescent cultures were acquired and tested for use as biological tracers for reservoir characterization by small independent oil companies. Initially these bacterial cultures were fastidious to work with, but when we finally determined their critical growth parameters simple test variations were developed that could be routinely accomplished. The intensity of their luminescence is easily distinguished by the human eye and requires no sophisticated technical knowledge or instrumentation. Cultures were received from culture banks and collected from marine environments. In our laboratory they were screened using the criteria of optimum growth and luminescence. Three stock cultures proved to grow profusely even when variations were made in nutrient additions, salts, and temperature. These three selected cultures were not inhibited when introduced to formations and formation waters and were not overgrown by other bacteria. Cultures isolated from the Gulf of Mexico were overgrown by indigenous bacteria and therefore, they were eliminated from further screening and adaption. Experiments were performed according to three major task descriptions: 1. Establish growth and luminescencing limitations of selected bacteria in various media, varying salt concentration and temperature. 2. Adapt cultures to formation waters. 3. Determine transport limitations of bioluminescent bacteria through representative reservoir cores. 19 refs., 5 figs.,more » 7 tabs.« less
Authors:
Publication Date:
OSTI Identifier:
6221773
Report Number(s):
DOE/BC/14666-5
ON: DE92001002
DOE Contract Number:
AC22-90BC14666
Resource Type:
Technical Report
Research Org:
Hardin-Simmons Univ., Abilene, TX (United States). Fairleigh Dickinson Labs.
Sponsoring Org:
DOE; USDOE, Washington, DC (United States)
Country of Publication:
United States
Language:
English
Subject:
02 PETROLEUM; 59 BASIC BIOLOGICAL SCIENCES; BACTERIA; LUMINESCENCE; PETROLEUM; ENHANCED RECOVERY; CULTURE MEDIA; PROGRESS REPORT; RESERVOIR ROCK; SANDSTONES; TRACER TECHNIQUES; DOCUMENT TYPES; ENERGY SOURCES; FOSSIL FUELS; FUELS; ISOTOPE APPLICATIONS; MICROORGANISMS; RECOVERY; ROCKS; SEDIMENTARY ROCKS 020300* -- Petroleum-- Drilling & Production; 550700 -- Microbiology