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Title: Improved method for detection of starch hydrolysis

A new starch hydrolysis detection method which does not rely on iodine staining or the use of color-complexed starch is described. A linear relationship was obtained with agar-starch plates when net clearing zones around colonies of yeasts were plotted against enzyme levels (semilogarithm scale) produced by the same yeast strains in liquid medium. A similar relationship between starch clearing zones and alpha-amylase levels from three different sources was observed. These observations suggest that the method is useful in mutant isolations, strain improvement programs, and the prediction of alpha-amylase activities in culture filtrates or column effluents. (Refs. 18).
Authors:
; ; ;
Publication Date:
OSTI Identifier:
6220653
Resource Type:
Journal Article
Resource Relation:
Journal Name: Appl. Environ. Microbiol.; (United States); Journal Volume: 44:3
Research Org:
Agricultural Microbiology Section, Dairy and Food Science Dept, Univ of Saskatchewan, Saskatoon, Canada S7N OWO
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; STARCH; HYDROLYSIS; AMYLASE; ENZYME ACTIVITY; ENZYMES; INDICATORS; MUTANTS; YEASTS; CARBOHYDRATES; CHEMICAL REACTIONS; DECOMPOSITION; FUNGI; GLYCOSYL HYDROLASES; HYDROLASES; LYSIS; MICROORGANISMS; O-GLYCOSYL HYDROLASES; ORGANIC COMPOUNDS; PLANTS; POLYSACCHARIDES; REAGENTS; SACCHARIDES; SOLVOLYSIS 140504* -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989); 090222 -- Alcohol Fuels-- Preparation from Wastes or Biomass-- (1976-1989)