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Title: Genomic clone encoding the. cap alpha. chain of the OKM1, LFA-1, and platelet glycoprotein IIb-IIIa molecules

LFA-1, an antigen involved in cytolytic T lymphocyte-mediated killing, and Mac-1, the receptor for complement component C3bi, constitute a family of structurally and functionally related cell surface glycoproteins involved in cellular interactions. In both mouse and man, Mac-1 (OKM1) and LFA-1 share a common 95-kDa ..beta.. subunit but are distinguished by their ..cap alpha.. chains, which have different cellular distributions, apparent molecular masses (165 and 177 kDa, respectively), and peptide maps. The authors report the isolation of a genomic clone from a human genomic library that on transfection into mouse fibroblasts produced a molecule(s) reactive with monoclonal antibodies to OKM1, to LFA-1, and to platelet glycoprotein IIb-IIIa. This gene was cloned by several cycles of transfection of L cells with a human genomic library cloned in lambda phase Charon 4A and subsequent rescue of the lambda phage. Transfection with the purified recombinant lambda DNA yielded a transfectant that expressed the three human ..cap alpha.. chains of OKM1, LFA-1, and glycoprotein IIb-IIIa, presumably in association with the murine ..beta.. chain.
Authors:
; ; ;
Publication Date:
OSTI Identifier:
6220523
Resource Type:
Journal Article
Resource Relation:
Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States); Journal Volume: 83:3
Research Org:
Univ. of Melbourne, Parkville, Australia
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GLUCOPROTEINS; GENETICS; MONOCLONAL ANTIBODIES; RADIORECEPTOR ASSAY; RECOMBINANT DNA; DNA-CLONING; ANTIGENS; BACTERIOPHAGES; CELL KILLING; COMPLEMENT; GENE REGULATION; IODINE 125; LYMPHOCYTES; MAN; MICE; RECEPTORS; ANIMAL CELLS; ANIMALS; ANTIBODIES; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BIOLOGY; BLOOD; BLOOD CELLS; BODY FLUIDS; CARBOHYDRATES; CLONING; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; DNA; ELECTRON CAPTURE RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LEUKOCYTES; MAMMALS; MATERIALS; MEMBRANE PROTEINS; MICROORGANISMS; NUCLEI; NUCLEIC ACIDS; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PARASITES; PRIMATES; PROTEINS; RADIOISOTOPES; RODENTS; SACCHARIDES; SOMATIC CELLS; TRACER TECHNIQUES; VERTEBRATES; VIRUSES 550201* -- Biochemistry-- Tracer Techniques