Detection of Listeria monocytogenes by using the polymerase chain reaction
Journal Article
·
· Applied and Environmental Microbiology; (USA)
OSTI ID:6217465
- Univ. of Colorado School of Medicine, Denver (USA)
A method was developed for detection of Listeria monocytogens by polymerase chain reaction amplification followed by agarose gel electrophoresis or dot blot analysis with {sup 32}P-labeled internal probe. The technique identified 95 of 95 L. monocytogenes strains, 0 of 12 Listeria strains of other species, and 0 of 12 non-Listeria strains.
- OSTI ID:
- 6217465
- Journal Information:
- Applied and Environmental Microbiology; (USA), Vol. 56:9; ISSN 0099-2240
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
DNA POLYMERASES
BIOASSAY
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DNA HYBRIDIZATION
ELECTROPHORESIS
GENE AMPLIFICATION
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BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
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550401* - Genetics- Tracer Techniques
DNA POLYMERASES
BIOASSAY
PATHOGENS
DNA HYBRIDIZATION
ELECTROPHORESIS
GENE AMPLIFICATION
PHOSPHORUS 32
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ENZYMES
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
NUCLEI
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADIOISOTOPES
TRANSFERASES
550401* - Genetics- Tracer Techniques