Identification of high- and low-affinity NGF receptors during development of the chicken central nervous system
- Cornell Univ. Medical College, New York, NY (USA)
In order to study regulation of the nerve growth factor (NGF) receptor during embryogenesis in chick brain, we have used affinity crosslinking of tissues with {sup 125}I-NGF. NGF interacts with high- and low-affinity receptors; high-affinity receptors are required for the majority of NGF's actions. Most measurements of receptor levels do not distinguish between high- and low-affinity forms of the receptor. We have used the lipophilic crosslinking agent HSAB to identify the high-affinity, functional receptor during development of the chicken central nervous system. A peak of expression during Embryonic Days 5-10 was detected in all regions of the chicken central nervous system, but, shortly after birth, only the cerebellar region displays significant levels of NGF receptor protein. The time course of expression confirms the dramatic regulation of the NGF receptor gene during defined embryonic periods. The detection of high-affinity NGF receptors in brain and neural retina provides strong evidence that NGF is involved in essential ontogenetic events in the development of the chicken central nervous system.
- OSTI ID:
- 6215289
- Journal Information:
- Developmental Biology; (USA), Vol. 142:2; ISSN 0012-1606
- Country of Publication:
- United States
- Language:
- English
Similar Records
Biochemical and biological properties of the nerve growth factor receptor
Pharmacology and function of melatonin receptors
Related Subjects
BRAIN
ONTOGENESIS
GROWTH FACTORS
RECEPTORS
GENES
AFFINITY
BIOCHEMICAL REACTION KINETICS
CHICKENS
CROSS-LINKING
IODINE 125
NERVES
REAGENTS
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIRDS
BODY
CENTRAL NERVOUS SYSTEM
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FOWL
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MEMBRANE PROTEINS
MITOGENS
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
POLYMERIZATION
PROTEINS
RADIOISOTOPES
REACTION KINETICS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques