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Title: Identification of a GTP-binding protein. cap alpha. subunit that lacks an apparent ADP-ribosylation site for pertussis toxin

Recent molecular cloning of cDNA for the ..cap alpha.. subunit of bovine transducin (a guanine nucleotide-binding regulatory protein, or G protein) has revealed the presence of two retinal-specific transducins, called T/sub r/ and T/sub c/, which are expressed in rod or cone photoreceptor cells. In a further study of G-protein diversity and signal transduction in the retina, the authors have identified a G-protein ..cap alpha.. subunit, which they refer to as G/sub z/..cap alpha.., by isolating a human retinal cDNA clone that cross-hybridizes at reduced stringency with bovine T/sub r/ ..cap alpha..-subunit cDNA. The deduced amino acid sequence of G/sub z/..cap alpha.. is 41-67% identical with those of other known G-protein ..cap alpha.. subunits. However, the 355-residue G/sub z/..cap alpha.. lacks a consensus site for ADP-ribosylation by pertussis toxin, and its amino acid sequence varies within a number of regions that are strongly conserved among all of the other G-protein ..cap alpha.. subunits. They suggest that G/sub z/..cap alpha.., which appears to be highly expressed in neural tissues, represents a member of a subfamily of G proteins that mediate signal transduction in pertussis toxin-insensitive systems.
Authors:
; ; ;
Publication Date:
OSTI Identifier:
6208506
Resource Type:
Journal Article
Resource Relation:
Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States); Journal Volume: 85:9
Research Org:
California Institute of Technology, Pasadena (USA)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; MESSENGER-RNA; HYBRIDIZATION; PROTEINS; AMINO ACID SEQUENCE; RECOMBINANT DNA; DNA SEQUENCING; RETINA; BIOCHEMISTRY; DNA-CLONING; GUANINE; MAN; MICE; NUCLEOTIDES; PHOSPHORUS 32; TOXINS; AMINES; ANIMALS; ANTIGENS; AROMATICS; AZAARENES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; BODY AREAS; CHEMISTRY; CLONING; DAYS LIVING RADIOISOTOPES; DNA; DNA HYBRIDIZATION; EYES; FACE; HEAD; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; ISOTOPES; LIGHT NUCLEI; MAMMALS; MATERIALS; MOLECULAR STRUCTURE; NUCLEI; NUCLEIC ACIDS; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PHOSPHORUS ISOTOPES; PRIMATES; PURINES; RADIOISOTOPES; RNA; RODENTS; SENSE ORGANS; STRUCTURAL CHEMICAL ANALYSIS; TOXIC MATERIALS; VERTEBRATES 550201* -- Biochemistry-- Tracer Techniques