Pertussis toxin inhibits somatostatin-induced K/sup +/ conductance in human pituitary tumor cells

Journal Article · Wed Jul 01 00:00:00 EDT 1987 · Am. J. Physiol.; (United States)

OSTI ID:6207940

Yamashita, N; Kojima, I; Shibuya, N; Ogata, E

The effect of pertussis toxin on somatostatin-induced K/sup +/ current was examined in dissociated human pituitary tumor cells obtained from two acromegalic patients. Somatostatin-induced hyperpolarization or K/sup +/ current was observed in 20 of 23 cells in adenoma 1 and 10 of 11 cells in adenoma 2. After treatment with pertussis toxin for 24 h, these responses were completely suppressed (0/14 in adenoma, 1, 0/10 in adenoma 2). Spontaneous action potentials, K/sup +/, Na/sup +/, and Ca/sup 2 +/ currents were well preserved after pertussis toxin treatment. When crude membrane fraction was incubated with (/sup 32/P)NAD, a 41K protein was ADP-ribosylated by pertussis toxin. Hormone release was inhibited by somatostatin and this inhibition was blocked by pertussis toxin treatment.

• Research Organization: Univ. of Tokyo School of Medicine (Japan)
• OSTI ID: 6207940
• Journal Information: Am. J. Physiol.; (United States), Vol. 253:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL MEMBRANES
BIOELECTRICITY
SOMATOSTATIN
RADIOIMMUNOASSAY
TOXINS
BIOLOGICAL EFFECTS
CATIONS
ELECTROPHORESIS
MAN
NAD
PHOSPHORUS 32
PITUITARY GLAND
POTASSIUM COMPOUNDS
PROTEINS
TUMOR CELLS
ALKALI METAL COMPOUNDS
ANIMAL CELLS
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
CHARGED PARTICLES
COENZYMES
DAYS LIVING RADIOISOTOPES
ELECTRICITY
ENDOCRINE GLANDS
GLANDS
IMMUNOASSAY
IMMUNOLOGY
IONS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MATERIALS
MEMBRANES
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PRIMATES
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
TOXIC MATERIALS
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques