Removal of phenols from wastewater by soluble and immobilized tyrosinase
- National Inst. for Resources and Environment, Ibaraki (Japan)
An enzymatic method for removal of phenols from industrial wastewater was investigated. Phenols in an aqueous solution were removed after treatment with mushroom tyrosinase. The reduction order of substituted phenols is catechol > p-cresol > p-chlorophenol > phenol > p-methoxyphenol. In the treatment of tyrosinase alone, no precipitate was formed but a color change from colorless to dark-brown was observed. The colored products were removed by chitin and chitosan which are available abundantly as shellfish waste. In addition, the reduction rate of phenols was observed to be accelerated in the presence of chitosan. Tyrosinase, immobilized by using amino groups in the enzyme on cation exchange resins, can be used repeatedly. By treatment with immobilized tyrosinase, 100% of phenol was removed after 2 h, and the activity was reduced very little even after 10 repeat treatments.
- OSTI ID:
- 6206327
- Journal Information:
- Biotechnology and Bioengineering; (United States), Vol. 42:7; ISSN 0006-3592
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
PHENOLS
MATERIALS RECOVERY
TYROSINASE
ENZYME ACTIVITY
WASTE WATER
DECONTAMINATION
IMMOBILIZED ENZYMES
INDUSTRIAL WASTES
AROMATICS
CLEANING
ENZYMES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
HYDROXYLASES
LIQUID WASTES
MANAGEMENT
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PROCESSING
PROTEINS
WASTE MANAGEMENT
WASTE PROCESSING
WASTES
WATER
540320* - Environment
Aquatic- Chemicals Monitoring & Transport- (1990-)
560300 - Chemicals Metabolism & Toxicology