Development of an enzyme membrane reactor for treatment of cyanide-containing wastewaters from the food industry

Journal Article · Sat Feb 20 00:00:00 EST 1993 · Biotechnology and Bioengineering; (United States)

DOI:https://doi.org/10.1002/bit.260410410· OSTI ID:6200665

Basheer, S; Kut, O M; Prenosil, J E; Bourne, J R ^[1]

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1. Swiss Federal Inst. of Tech., Zurich (Switzerland)

Cyanidase, an immobilized enzyme preparation for hydrolyzing cyanide to ammonia and formate, was applied for the treatment of cyanide-containing waste-waters from the food industry. Apricot seed extract was chosen as a model effluent. The enzymatic hydrolysis of pure amygdalin, the main cyanogenic glycoside in the extract, and the degradation of the cyanide formed was investigated and compared with the behavior of the real extract in a batch slurry reactor. A diffusional-type, flat-membrane reactor with immobilized cyanidase was developed, where the enzyme is effectively protected from adverse effects of high molecular components contained in the extract. For monitoring continuous-membrane reactor operation, a new unsegmented ammonia measurement system was developed and applied. In continuous operation the cyanidase retained its original activity for more than 400 hours on stream.

• OSTI ID: 6200665
• Journal Information: Biotechnology and Bioengineering; (United States), Vol. 41:4; ISSN 0006-3592
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CYANIDES
BIODEGRADATION
GLYCOSIDES
ENZYMATIC HYDROLYSIS
WASTE WATER
DECONTAMINATION
BIOREACTORS
FOOD INDUSTRY
GLUCOSIDASE
IMMOBILIZED ENZYMES
WATER POLLUTION
CARBOHYDRATES
CHEMICAL REACTIONS
CLEANING
DECOMPOSITION
ENZYMES
GLYCOSYL HYDROLASES
HYDROGEN COMPOUNDS
HYDROLASES
HYDROLYSIS
INDUSTRY
LIQUID WASTES
LYSIS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
POLLUTION
PROTEINS
SOLVOLYSIS
WASTES
WATER
540320* - Environment
Aquatic- Chemicals Monitoring & Transport- (1990-)
560300 - Chemicals Metabolism & Toxicology