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Title: Psoralen-plus-light damage and repair in transforming DNA of Bacillus subtilis

The relative contributions of excision and recombination in the repair of damage by 8-methoxypsoralen (8-MOP) plus black light to Bacillus subtilis were studied. The results indicate that the pyrimidine dimer excision system and a recombination pathway are probably both involved in repair of lethal damage to cells exposed in vivo to 8-MOP plus black light, but repair is not very efficient. Transforming DNA exposed in vitro to 8-MOP plus black light was inactivated mainly by crosslinks rather than by monoadducts, and was repaired predominantly by an incision-dependent process. There was very little demonstrable damage-induced recombination in transforming DNA.
Authors:
Publication Date:
OSTI Identifier:
6196010
Report Number(s):
CONF-810694-1
ON: DE81024140
DOE Contract Number:
W-7405-ENG-26
Resource Type:
Conference
Resource Relation:
Conference: 25. Wind River conference on genetic exchange, Estes Park, CO, USA, 8 Jun 1981
Research Org:
Oak Ridge National Lab., TN (USA); Tennessee Univ., Oak Ridge (USA). Graduate School of Biomedical Sciences
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; BIOLOGICAL REPAIR; BIOLOGICAL PATHWAYS; COUMARINS; RADIOSENSITIVITY EFFECTS; DNA; BACILLUS SUBTILIS; BIOLOGICAL RADIATION EFFECTS; CROSS-LINKING; IN VIVO; IRRADIATION; ULTRAVIOLET RADIATION; ANTICOAGULANTS; BACILLUS; BACTERIA; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; CHEMICAL REACTIONS; DRUGS; ELECTROMAGNETIC RADIATION; HEMATOLOGIC AGENTS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; POLYMERIZATION; RADIATION EFFECTS; RADIATIONS; RECOVERY; REPAIR 560112* -- Radiation Effects on Biochemicals-- In Microorganisms-- (-1987); 560131 -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)