alpha-Ketobutyrate metabolism in perfused rat liver: regulation of alpha-ketobutyrate decarboxylation and effects of alpha-ketobutyrate on pyruvate dehydrogenase
Abstract
The oxidative decarboxylation and subsequent production of glucose from alpha-ketobutyrate were studied using perfused livers from fasted rats. The production of /sup 14/CO/sub 2/ from alpha-keto-(1-/sup 14/C)butyrate increased monotonically while the production of glucose from alpha-ketobutyrate was biphasic as the perfusate concentration of alpha-ketobutyrate was increased. The biphasic gluconeogenic response using alpha-ketobutyrate as the gluconeogenic precursor was similar to that observed with propionate. The effects of alpha-ketobutyrate infusion on pyruvate decarboxylation were tested and it was found that at low perfusate pyruvate concentrations increasing alpha-ketobutyrate led to increasing inhibition of pyruvate decarboxylation, while at high perfusate pyruvate concentrations an initial inhibition was apparent which did not increase substantially with increasing alpha-ketobutyrate concentrations. The results obtained indicate that the regulation of alpha-ketobutyrate metabolism by oxidative decarboxylation differs significantly from that of pyruvate. In addition, while the rate of gluconeogenesis using alpha-ketobutyrate as a precursor was remarkably similar to that using propionate as a gluconeogenic precursor, the effects of alpha-ketobutyrate on the oxidative decarboxylation of pyruvate were qualitatively different from the effects of propionate on pyruvate metabolism.
- Authors:
- Publication Date:
- Research Org.:
- Univ. of Texas Health Science Center, San Antonio
- OSTI Identifier:
- 6194400
- Resource Type:
- Journal Article
- Journal Name:
- Arch. Biochem. Biophys.; (United States)
- Additional Journal Information:
- Journal Volume: 2
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; CARBON 14 COMPOUNDS; DECARBOXYLATION; CARBOXYLIC ACID SALTS; LIVER; KINETICS; OXIDOREDUCTASES; BIOCHEMISTRY; CARBON DIOXIDE; CARNITINE; RATS; AMINO ACIDS; ANIMALS; BODY; CARBON COMPOUNDS; CARBON OXIDES; CARBOXYLIC ACIDS; CHALCOGENIDES; CHEMICAL REACTIONS; CHEMISTRY; DIGESTIVE SYSTEM; ENZYMES; GLANDS; HYDROXY ACIDS; LABELLED COMPOUNDS; MAMMALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; OXIDES; OXYGEN COMPOUNDS; RODENTS; VERTEBRATES; VITAMIN B GROUP; VITAMINS; 550201* - Biochemistry- Tracer Techniques
Citation Formats
Lapointe, D S, and Olson, M S. alpha-Ketobutyrate metabolism in perfused rat liver: regulation of alpha-ketobutyrate decarboxylation and effects of alpha-ketobutyrate on pyruvate dehydrogenase. United States: N. p., 1985.
Web. doi:10.1016/0003-9861(85)90226-7.
Lapointe, D S, & Olson, M S. alpha-Ketobutyrate metabolism in perfused rat liver: regulation of alpha-ketobutyrate decarboxylation and effects of alpha-ketobutyrate on pyruvate dehydrogenase. United States. https://doi.org/10.1016/0003-9861(85)90226-7
Lapointe, D S, and Olson, M S. 1985.
"alpha-Ketobutyrate metabolism in perfused rat liver: regulation of alpha-ketobutyrate decarboxylation and effects of alpha-ketobutyrate on pyruvate dehydrogenase". United States. https://doi.org/10.1016/0003-9861(85)90226-7.
@article{osti_6194400,
title = {alpha-Ketobutyrate metabolism in perfused rat liver: regulation of alpha-ketobutyrate decarboxylation and effects of alpha-ketobutyrate on pyruvate dehydrogenase},
author = {Lapointe, D S and Olson, M S},
abstractNote = {The oxidative decarboxylation and subsequent production of glucose from alpha-ketobutyrate were studied using perfused livers from fasted rats. The production of /sup 14/CO/sub 2/ from alpha-keto-(1-/sup 14/C)butyrate increased monotonically while the production of glucose from alpha-ketobutyrate was biphasic as the perfusate concentration of alpha-ketobutyrate was increased. The biphasic gluconeogenic response using alpha-ketobutyrate as the gluconeogenic precursor was similar to that observed with propionate. The effects of alpha-ketobutyrate infusion on pyruvate decarboxylation were tested and it was found that at low perfusate pyruvate concentrations increasing alpha-ketobutyrate led to increasing inhibition of pyruvate decarboxylation, while at high perfusate pyruvate concentrations an initial inhibition was apparent which did not increase substantially with increasing alpha-ketobutyrate concentrations. The results obtained indicate that the regulation of alpha-ketobutyrate metabolism by oxidative decarboxylation differs significantly from that of pyruvate. In addition, while the rate of gluconeogenesis using alpha-ketobutyrate as a precursor was remarkably similar to that using propionate as a gluconeogenic precursor, the effects of alpha-ketobutyrate on the oxidative decarboxylation of pyruvate were qualitatively different from the effects of propionate on pyruvate metabolism.},
doi = {10.1016/0003-9861(85)90226-7},
url = {https://www.osti.gov/biblio/6194400},
journal = {Arch. Biochem. Biophys.; (United States)},
number = ,
volume = 2,
place = {United States},
year = {Fri Nov 01 00:00:00 EST 1985},
month = {Fri Nov 01 00:00:00 EST 1985}
}