Immunogenicity of ascites tumor cells following in vitro hyperthermia
The concept that host immunization may be achieved by heat-induced antigenic modifications of cancer cells and/or the release of immunogenic products by dead or dying tumor cells following in vitro heating was examined. Ehrlich ascites cells were used, inasmuch as it was claimed that in vitro hyperthermia increased the immunogenicity of these cells. Tumor cell populations of different viability were obtained by heating Ehrlich cells at 42.5 degrees, 45 degrees, or 60 degrees C. Viable and nonviable cells were separated by Ficoll-Hypaque density centrifugation; viable nonreplicating cells were obtained by treatment with mitomycin C. Cell populations of different viability after heating were left to die slowly over 3 days at 37 degrees C. Swiss TO mice were then given injections of the treated cells and/or medium. No survival benefit occurred in mice inoculated with any of these different components and then challenged with viable tumor cells. Injection of irradiated cells, however, did produce host immunity. Similarly, D23 rat hepatoma ascites cells produced host immunity after 15,000 rad but not after heating. The claim that in vitro hyperthermia increases the immunogenicity of tumor cells was not confirmed.
- Research Organization:
- University Department of Clinical Biochemistry, Royal Victoria Infirmary, Newcastle upon Tyne, England
- OSTI ID:
- 6187260
- Journal Information:
- NIDA Res. Monogr.; (United States), Vol. 61
- Country of Publication:
- United States
- Language:
- English
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ASCITES TUMOR CELLS
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SURVIVAL CURVES
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ANIMAL CELLS
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560121* - Radiation Effects on Cells- External Source- (-1987)