Degradation of extracellular matrix by larvae of Schistosoma mansoni. I. Degradation by cercariae as a model for initial parasite invasion of host
The ability of cercariae of Schistosoma mansoni to degrade a model extracellular connective tissue matrix produced by rat vascular smooth muscle cells in culture was investigated. In this model, connective tissue macromolecules are present in the interactive framework that characterizes their structure in vivo. Cercariae were stimulated to degrade the matrix by skin lipid or linoleic acid. At the maximally stimulating concentration of linoleic acid (25 ..mu..g/cm/sup 2/), 68% of the total matrix was degraded, including 57% of the glycoprotein, 79% of the elastin, and 8% of the collagen. Degradation of matrix was inhibited by ..cap alpha../sub 1/-proteinase inhibitor and soybean trypsin inhibitor. Ethylenediaminetetraacetic acid inhibited degradation by unstimulated but not linoleic acid-stimulated cercariae. Preacetabular gland secretions collected from cercariae also degraded the matrix with an activity 86% of that of live cercariae. Preacetabular gland proteolytic activity was also inhibited by ..cap alpha../sub 1/-proteinase inhibitor, soybean trypsin inhibitor, and ethylenediaminetetraacetic acid. The similar characteristics of matrix degradation by both live cercariae and cercarial preacetabular gland secretions support the idea that a proteinase secreted from cercarial preacetabular glands facilitates invasion of skin and connective tissue by these larvae. Degradation of elastin and glycoprotein constituentes of extracellular matrix is probably essential for skin penetration.
- Research Organization:
- Univ. of California, San Francisco
- DOE Contract Number:
- AC03-76SF01012
- OSTI ID:
- 6184590
- Journal Information:
- Lab. Invest.; (United States), Vol. 49:2
- Country of Publication:
- United States
- Language:
- English
Similar Records
PARASITE MIGRATION AND HOST REACTION IN MICE EXPOSED TO IRRADIATED CERCARIAE OF SCHISTOSOMA MANSONI
Degradation of connective tissue matrices by macrophages. II. Influence of matrix composition on proteolysis of glycoproteins, elastin, and collagen by macrophages in culture
Related Subjects
CONNECTIVE TISSUE
PROTEOLYSIS
LARVAE
SERINE PROTEINASES
ENZYME ACTIVITY
BIOLOGICAL MODELS
ENZYME INHIBITORS
LINOLEIC ACID
METAMORPHOSIS
RATS
RESPONSE MODIFYING FACTORS
SCHISTOSOMA
SKIN
ANIMAL TISSUES
ANIMALS
BODY
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMES
HELMINTHS
HYDROLASES
MAMMALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
PLATYHELMINTHS
RODENTS
TISSUES
TREMATODES
VERTEBRATES
550200* - Biochemistry