Recent advances in X-ray microanalysis in dermatology
Electron microprobe and proton microprobe X-ray analysis can be used in several areas of dermatological research. With a proton probe, the distribution of trace elements in human hair can be determined. Electron microprobe analysis on freeze-dried cryosections of guinea-pig and human epidermis shows a marked gradient of Na, P and K over the stratum granulosum. In sections of freeze-substituted human skin this gradient is less steep. This difference is likely to be due to a decrease in water content of the epidermis towards the stratum corneum. Electron microprobe analysis of the epidermis can, for analysis of trace elements, be complemented by the proton microprobe. Quantitative agreement between the two techniques can be obtained by the use of a standard. Proton microprobe analysis was used to determine the distribution of Ni or Cr in human epidermis exposed to nickel or chromate ions. Possible differences in water content between the stratum corneum of patients with atopic eczema and normal stratum corneum was investigated in skin freeze-substituted with Br-doped resin. No significant differences were observed.
- Research Organization:
- Karolinska Institute, Stockholm, Sweden
- OSTI ID:
- 6180803
- Journal Information:
- Scanning Electron Microsc.; (United States), Journal Name: Scanning Electron Microsc.; (United States)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ELEMENTS
TISSUE DISTRIBUTION
SKIN
ELECTRON MICROPROBE ANALYSIS
PROTON MICROPROBE ANALYSIS
CHROMATES
EPIDERMIS
GUINEA PIGS
HAIR
NICKEL COMPOUNDS
PHOSPHORUS COMPOUNDS
POTASSIUM COMPOUNDS
PSORIASIS
SKIN DISEASES
TRACE AMOUNTS
ALKALI METAL COMPOUNDS
ANIMAL TISSUES
ANIMALS
BODY
CHEMICAL ANALYSIS
CHROMIUM COMPOUNDS
DISEASES
DISTRIBUTION
EPITHELIUM
MAMMALS
MICROANALYSIS
NONDESTRUCTIVE ANALYSIS
ORGANS
OXYGEN COMPOUNDS
RODENTS
TISSUES
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics