Use of laser-UV for inactivation of virus in blood products

Journal Article · Sat Aug 01 00:00:00 EDT 1987 · Blood; (United States)

OSTI ID:6176545

Prodouz, K N; Fratantoni, J C; Boone, E J; Bonner, R F

Inactivation of virus by UV radiation was examined as a potential method for sterilization of blood products. Samples of attenuated poliovirus, platelets and plasma were uniformly irradiated with a XeCl excimer laser that delivered 40 nsec pulses of UV at 308 nm (UVB308). Intensities and exposure does were varied from 0.11 to 1.40 MW/cm2 and 0.51 to 56.0 J/cm2, respectively. In studies conducted with low intensity UVB308 (less than or equal to 0.17 MW/cm2), using exposure doses greater than or equal to 10.8 J/cm2, it was possible to inactivate poliovirus by 4 to 6 log10. Platelets irradiated with doses less than or equal to 21.5 J/cm2 exhibited minimal damage as assessed by aggregation activity and spontaneous release of serotonin. Examination of the coagulation activity of irradiated plasma indicated that exposure doses less than or equal to 21.5 J/cm2 resulted in less than 20% increase in prothrombin and partial thromboplastin times. The use of UVB308 at a higher intensity (1.4 MW/cm2) over a similar range of exposure doses did not enhance viral inactivation but did result in increased damage to platelet and plasma proteins. These results demonstrate that at 308 nm there exists a window of efficacy for exposure doses between 10.8 and 21.5 J/cm2 and peak intensities less than or equal to 0.17 MW/cm2 in which a hardy virus is significantly inactivated and platelets and plasma proteins are, by functional criteria, minimally affected. Increased viral inactivation cannot be accomplished with higher UV intensities and will require additional or alternate measures.

• Research Organization: Center for Drugs and Biologics, Food and Drug Administration, Bethesda, MD
• OSTI ID: 6176545
• Journal Information: Blood; (United States), Vol. 70:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BLOOD PLASMA
BIOLOGICAL RADIATION EFFECTS
RADIOSTERILIZATION
BLOOD PLATELETS
POLIO VIRUS
BLOOD
BLOOD COAGULATION
DOSE-RESPONSE RELATIONSHIPS
LASERS
RADIATION DOSES
SEROTONIN
AMINES
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZOLES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD CELLS
BODY FLUIDS
DOSES
DRUGS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INDOLES
MATERIALS
MICROORGANISMS
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
PYRROLES
RADIATION EFFECTS
RADIOPROTECTIVE SUBSTANCES
STERILIZATION
SYMPATHOMIMETICS
TRYPTAMINES
VIRUSES
560130* - Radiation Effects on Microorganisms