Expression of striatal D sub 1 dopamine receptors coupled to inositol phosphate production and Ca sup 2+ mobilization in Xenopus oocytes

Journal Article · Thu Mar 01 00:00:00 EST 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.6.2196· OSTI ID:6172230

Mahan, L C; Monsma, Jr, F J; Sibley, D R ^[1]; Burch, R M ^[2]

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1. National Institutes of Health, Bethesda, MD (USA)
2. Nova Pharmaceutical Corp., Baltimore, MD (USA)

Expression of central nervous system receptors for dopamine was examined by injection of poly(A){sup +} RNA (mRNA) from rat striatum into oocytes from Xenopus laevis. Electrophysiological measurements in mRNA-injected oocytes indicated that addition of 100 {mu}M dopamine induced an inward current that was consistent with the activation of endogenous Ca{sup 2+}-dependent Cl{sup {minus}} channels. This current was also elicited by addition of the selective D{sub 1} agonist SKF 38393 but not by the selective D{sub 2} agonist quinpirole. Using {sup 45}Ca{sup 2+} efflux assays, addition of 100 {mu}M dopamine to injected oocytes stimulated efflux 2- to 3-fold. This increase was mimicked by SKF 38393 and was blocked by the D{sub 1}-selective antagonist (+)SCH 23390 but not by the D{sub 2}-selective antagonist domperidone. No increase in {sup 45}Ca{sup 2+} efflux was seen with 100 {mu}M quinpirole. Size fractionation of striatal mRNA yielded a single peak (2.5-3.0 kilobases) of D{sub 1} receptor-mediated {sup 45}Ca{sup 2+} efflux activity in injected oocytes. Thus, in addition to D{sub 1} receptors that stimulate adenylyl cyclase, rat striatum contains D{sub 1} receptors that can couple to InsP{sub 3} formation and mobilization of intracellular Ca{sup 2+}.

• OSTI ID: 6172230
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 87:6; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CALCIUM 45
MEMBRANE TRANSPORT
CYCLASES
ENZYME INDUCTION
DOPAMINE
RECEPTORS
AMPHIBIANS
CELL MEMBRANES
INOSITOLS
MESSENGER-RNA
OOCYTES
RATS
SEROTONIN
ALKALINE EARTH ISOTOPES
AMINES
ANIMALS
AQUATIC ORGANISMS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CALCIUM ISOTOPES
CARBOHYDRATES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DRUGS
ENZYMES
EVEN-ODD NUCLEI
GENE REGULATION
GERM CELLS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INDOLES
INTERMEDIATE MASS NUCLEI
ISOTOPES
LYASES
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
MONOSACCHARIDES
NEUROREGULATORS
NUCLEI
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENOLS
POLYPHENOLS
PROTEINS
PYRROLES
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
RNA
RODENTS
SACCHARIDES
SYMPATHOMIMETICS
TRYPTAMINES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques