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Title: Increased regional vascular albumin permeation in the rat during anaphylaxis

Abstract

The changes in vascular albumin permeation induced by systemic anaphylaxis were studied simultaneously in 21 different tissues of the same animal. Before Ag challenge sensitized rats were injected i.v. with 125I-albumin (test tracer), 51Cr-RBC (vascular space marker) and 57Co-EDTA (extravascular space marker). The index of vascular permeation used was the tissue to blood isotope ratio (tbir), which was obtained by dividing the ratio of 125I/51Cr counts in each tissue by the ratio of the same isotopes in the arterial blood sample. After Ag challenge, the increase in the tbir varied considerably among the different tissues. The most pronounced increase was noted in the lymph node (ninefold) followed by the aorta and mesentery (six- to sevenfold) and the various parts of the gastrointestinal tract (four- to sixfold). In the skin less than skeletal muscle less than lung less than liver and eye two- to fourfold increases occurred. Relatively minor increases in albumin permeation (less than twofold) were observed in the brain less than kidney less than heart and less than spleen. The testis was the only organ in which no significant change occurred. For some of the tissues there was also an increase in the tbir for 57Co/51Cr (an index ofmore » the extracellular fluid space) suggesting edema formation. The highest increase was noted in the aorta (fourfold). Minor increases occurred in the atrium of the heart, stomach, duodenum, and lymph nodes. There was also a 36% increase in hematocrit. Therefore, systemic anaphylaxis caused extensive extravasation of albumin and hemoconcentration.« less

Authors:
; ; ;
Publication Date:
Research Org.:
Washington Univ. School of Medicine, St. Louis, MO (USA)
OSTI Identifier:
6172222
Resource Type:
Journal Article
Journal Name:
J. Immunol.; (United States)
Additional Journal Information:
Journal Volume: 142:6
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ALBUMINS; TISSUE DISTRIBUTION; BLOOD VESSELS; PERMEABILITY; ALLERGY; ANAPHYLAXIS; BLOOD PRESSURE; CHROMIUM 51; COBALT 57; EDTA; IODINE 125; ISOTOPE RATIO; MICE; RATS; TRACER TECHNIQUES; AMINO ACIDS; ANIMALS; BETA DECAY RADIOISOTOPES; BODY; CARBOXYLIC ACIDS; CARDIOVASCULAR SYSTEM; CHELATING AGENTS; CHROMIUM ISOTOPES; COBALT ISOTOPES; DAYS LIVING RADIOISOTOPES; DISTRIBUTION; ELECTRON CAPTURE RADIOISOTOPES; EVEN-ODD NUCLEI; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PATHOLOGICAL CHANGES; PROTEINS; RADIOISOTOPES; RODENTS; VERTEBRATES; 550901* - Pathology- Tracer Techniques

Citation Formats

Leng, W, Chang, K, Williamson, J R, and Jakschik, B A. Increased regional vascular albumin permeation in the rat during anaphylaxis. United States: N. p., 1989. Web.
Leng, W, Chang, K, Williamson, J R, & Jakschik, B A. Increased regional vascular albumin permeation in the rat during anaphylaxis. United States.
Leng, W, Chang, K, Williamson, J R, and Jakschik, B A. 1989. "Increased regional vascular albumin permeation in the rat during anaphylaxis". United States.
@article{osti_6172222,
title = {Increased regional vascular albumin permeation in the rat during anaphylaxis},
author = {Leng, W and Chang, K and Williamson, J R and Jakschik, B A},
abstractNote = {The changes in vascular albumin permeation induced by systemic anaphylaxis were studied simultaneously in 21 different tissues of the same animal. Before Ag challenge sensitized rats were injected i.v. with 125I-albumin (test tracer), 51Cr-RBC (vascular space marker) and 57Co-EDTA (extravascular space marker). The index of vascular permeation used was the tissue to blood isotope ratio (tbir), which was obtained by dividing the ratio of 125I/51Cr counts in each tissue by the ratio of the same isotopes in the arterial blood sample. After Ag challenge, the increase in the tbir varied considerably among the different tissues. The most pronounced increase was noted in the lymph node (ninefold) followed by the aorta and mesentery (six- to sevenfold) and the various parts of the gastrointestinal tract (four- to sixfold). In the skin less than skeletal muscle less than lung less than liver and eye two- to fourfold increases occurred. Relatively minor increases in albumin permeation (less than twofold) were observed in the brain less than kidney less than heart and less than spleen. The testis was the only organ in which no significant change occurred. For some of the tissues there was also an increase in the tbir for 57Co/51Cr (an index of the extracellular fluid space) suggesting edema formation. The highest increase was noted in the aorta (fourfold). Minor increases occurred in the atrium of the heart, stomach, duodenum, and lymph nodes. There was also a 36% increase in hematocrit. Therefore, systemic anaphylaxis caused extensive extravasation of albumin and hemoconcentration.},
doi = {},
url = {https://www.osti.gov/biblio/6172222}, journal = {J. Immunol.; (United States)},
number = ,
volume = 142:6,
place = {United States},
year = {Wed Mar 15 00:00:00 EST 1989},
month = {Wed Mar 15 00:00:00 EST 1989}
}