Absorption of enzymatically active sup 125 I-labeled bovine milk xanthine oxidase fed to rabbits
- ICI Americas Inc., Wilmington, DE (USA)
- Univ. of Delaware, Newark (USA)
Rabbits fed a regular laboratory diet supplemented with a high-fat milk containing xanthine oxidase (XO) were studied to determine the presence of active XO in the blood. A pilot feeding study, where rabbits consumed a high-fat diet containing xanthine oxidase, showed a correlation between dairy food consumption and XO activity in the blood. Antibody to dietary XO was also found. In a second study, rabbits were fed ad libitum the high-fat milk and blood serum samples were tested weekly for XO activity. No elevation in serum XO activity was found. A third study showed that serum XO activity was increased when rabbits were force fed the high-fat milk. The final study consisted of force feeding {sup 125}I-labeled XO to one rabbit to ascertain whether the observed increase in serum XO was due to dietary or endogenous XO. Isoelectric focusing of sera collected from the test rabbit strongly suggested that at least a portion of the serum XO contained the radioactive label. This is the first direct evidence showing the uptake of dietary active XO from the gut.
- OSTI ID:
- 6172162
- Journal Information:
- Journal of Agricultural and Food Chemistry; (USA), Vol. 38:5; ISSN 0021-8561
- Country of Publication:
- United States
- Language:
- English
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BLOOD SERUM
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INTESTINAL ABSORPTION
IODINE 125
MILK
OXIDOREDUCTASES
TRACER TECHNIQUES
UPTAKE
XANTHINES
ABSORPTION
ANIMALS
AROMATICS
AZAARENES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BODY FLUIDS
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DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
FOOD
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
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MAMMALS
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NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PURINES
RADIOISOTOPES
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550201* - Biochemistry- Tracer Techniques