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Title: Platelet-derived growth factor agonist activity of a secreted form of the v-sis oncogene product

The authors have compared the functional properties of a growth factor partially purified from medium conditioned by simian sarcoma virus-transformed cells with those of platelet-derived growth factor (PDGF). The factor mimicked the effects induced by PDGF: it bound to and activated human fibroblast PDGF receptors and stimulated DNA synthesis. The factor behaved as a secretory protein, since about 95% of the receptor-binding activity was found in the medium after a 48-hr serum-free incubation. Structural characterization of the PDGF-like activity revealed a M/sub r/ 24,000 intracellular protein and two polypeptides of M/sub r/ 13,000 and 11,500 released into the medium. The M/sub r/ 13,000 component bound to human fibroblasts; this binding was competitively inhibited by PDGF. The data support the possibility that oncogene products may elicit transforming activity by interacting with the normal cellular mitogenic pathway.
Authors:
; ; ; ;
Publication Date:
OSTI Identifier:
6170969
Resource Type:
Journal Article
Resource Relation:
Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States); Journal Volume: 82:6
Research Org:
Univ. of Uppsala, Sweden
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BLOOD PLATELETS; RADIORECEPTOR ASSAY; PROTEINS; CHEMICAL PROPERTIES; MOLECULAR WEIGHT; ANIMAL GROWTH; DNA; GENES; HYBRIDIZATION; IODINE 125; MESSENGER-RNA; ONCOGENIC TRANSFORMATIONS; ONCOGENIC VIRUSES; PHOSPHORUS 32; SIMIAN VIRUS; SULFUR 35; TRITIUM COMPOUNDS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CELL TRANSFORMATIONS; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; EVEN-ODD NUCLEI; GROWTH; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LABELLED COMPOUNDS; LIGHT NUCLEI; MATERIALS; MICROORGANISMS; NUCLEI; NUCLEIC ACIDS; ODD-EVEN NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PARASITES; PHOSPHORUS ISOTOPES; RADIOISOTOPES; RNA; SULFUR ISOTOPES; TRACER TECHNIQUES; VIRUSES 550301* -- Cytology-- Tracer Techniques; 550201 -- Biochemistry-- Tracer Techniques