NMR analyses of the cold cataract. III. /sup 13/C acrylamide studies
/sup 13/C-enriched acrylamide was employed to further delineate the action of this compound in preventing the cold cataract phenomenon when it is incorporated (in vitro) into young human and rabbit lenses. The extent of acrylamide incorporation, in the dark and with concurrent UV exposure, was monitored by /sup 13/C NMR spectroscopy. These studies provide further evidence that UV exposure causes permanent acrylamide photobinding within the lens. In such lenses, the gamma crystallin fraction of the soluble lens proteins is affected to the greatest extent. It appears to become aggregated and/or combined with the alpha and beta fractions resulting in an apparent loss of most of the gamma monomers. There is also an age-related effect with respect to the amount of acrylamide that can be incorporated into the lens. The decrease in acrylamide incorporation with age directly parallels the age-related decline in gamma crystallin levels.
- Research Organization:
- Emory Univ., Atlanta, GA
- OSTI ID:
- 6168775
- Journal Information:
- Invest. Ophthalmol. Visual Sci.; (United States), Vol. 10
- Country of Publication:
- United States
- Language:
- English
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