Ethanol stimulates calcium mobilization, phosphoinositide turnover and shape change in human platelets
The effect of ethanol of cytosolic free calcium levels was investigated in human platelets which were loaded with the intracellular calcium indicator FURA-2. Administration of ethanol in the concentration range of 50-300 mM resulted in a transient, dose-dependent increase in cytosolic calcium, maximal within 5 seconds and returning to near basal levels over the next 5 minutes. Parallel incubations of platelets with the same concentrations of ethanol stimulated shape change as detected in an aggregometer. Chelation of external calcium with EGTA prior to the addition of ethanol reduced the calcium burst partially, but not completely, whereas shape change was largely unaffected. Addition of ethanol to /sup 32/P-labeled platelets resulted in a 16% decrease in the level of /sup 32/P-phosphatidylinositol 4,5-bisphosphate and a 14% increase in /sup 32/P-phosphatidylinositol 4-phosphate within 2 minutes. /sup 32/P-phosphatidic acid levels increased rapidly within 30 seconds and rose linearly thereafter. Phosphatidylinositol and phosphatidylcholine were unaffected by ethanol. The results indicate that ethanol mobilizes intracellular calcium by activation of phosphoinositide-specific phospholipase C, thereby stimulating platelet shape change.
- Research Organization:
- Thomas Jefferson Univ. School of Medicine, Philadelphia, PA
- OSTI ID:
- 6168624
- Report Number(s):
- CONF-870644-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BLOOD PLATELETS
MORPHOLOGICAL CHANGES
CALCIUM
MEMBRANE TRANSPORT
ETHANOL
BIOLOGICAL EFFECTS
PHOSPHOLIPIDS
SECRETION
DOSE-RESPONSE RELATIONSHIPS
EGTA
MAN
PHOSPHORUS 32
TRACER TECHNIQUES
ALCOHOLS
ALKALINE EARTH METALS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CHELATING AGENTS
DAYS LIVING RADIOISOTOPES
ELEMENTS
ESTERS
GLYCOLS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPIDS
MAMMALS
MATERIALS
METALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques