Pharmacological and biochemical properties of the benzodiazepine-GABA receptor in codfish brain in comparison with mammalian brain
The GABA receptor of codfish brain is encoded by an ancestral gene of the mammalian GABA receptor based on phylogenetic studies. The mammalian GABA receptor consists of at least two subunits ({beta} and {alpha}) which could be photoaffinity labeled by the GABA agonist ({sup 3}H)muscimol (57 kDa) and the benzodiazepine (BZ) agonist ({sup 3}H)flunitrazepam (52 kDa), respectively. In contrast, electrophoresis of codfish GABA receptor photoaffinity labeled by the same ligands showed a single radioactive peak on sodium dodecyl surface polyarcylamide gel, giving rise to a relative molecular weight of 56-57 kDa equivalent to the {beta} subunit of 57 kDa in mammals. The homogeneity of purified receptor using benzodiazepine (Ro 7-1986/1) affinity chromatography was further verified by two-dimensional gel electrophoresis based on isoelectric point and molecular weight, in addition to a single band on a silver stained gel and specific activity. The receptor density and affinity constant for ({sup 3}H)muscimol and ({sup 3}H)flunitrazepam are comparable to those in bovine, rate, and human brain.
- Research Organization:
- California Univ., Los Angeles, CA (USA)
- OSTI ID:
- 6159509
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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AMINOBUTYRIC ACID
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COMPARATIVE EVALUATIONS
LIGANDS
MAN
MOLECULAR WEIGHT
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
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AUTONOMIC NERVOUS SYSTEM AGENTS
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FISHES
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
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ORGANIC ACIDS
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550201* - Biochemistry- Tracer Techniques