Preparation of polyclonal antibodies against pig trachea apomucin

Conference · Fri May 01 00:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6156645

Lin, H H; Carlson, D M

Purified procine tracheal mucin (PTM) was deglycosylated by treatment with trifluoromethanesulfonic acid (TFMS). A preferrential release of fucose, galactose > GlcNAc > GalNAc was observed during the deglycosylation. After five hours of TFMS treatment at 5C, over 90% of the carbohydrate was released leaving mainly Ga1NAc attached to the core protein. The residual Ga1NAc was further removed by three series of the Smith Degradation. The resulting apomucin consisted mainly of (mol %) Thr, 14; Ser, 12; Gly, 12; Glx, 10; Ala, 9; Pro, 8; Val, 7; Asx, 7. Sugars were not detected by gas chromatography. Antibodies obtained by immunizing rabbits with the apomucin recognized only the apomucin and not the holomucin in a double-sandwich ELISA assay. The ELISA is highly specific and has detection limits in the ng range. This antibody preparation was used in precipitation assays of cell-free translation products of pig trachea epithelium RNAs. Translocation products labeled with (TH)Ser and (TH)Thr showed significantly higher counts than with control serum.

• Research Organization: Univ. of California, Davis
• OSTI ID: 6156645
• Report Number(s): CONF-870644-; TRN: 87-028683
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANTIBODIES
CHEMICAL PREPARATION
GLUCOPROTEINS
CHEMICAL COMPOSITION
METABOLISM
AMINO ACIDS
GAS CHROMATOGRAPHY
RADIOENZYMATIC ASSAY
SWINE
TRACER TECHNIQUES
TRACHEA
TRITIUM COMPOUNDS
ANIMALS
CARBOHYDRATES
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DOMESTIC ANIMALS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RESPIRATORY SYSTEM
SACCHARIDES
SEPARATION PROCESSES
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques