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Title: Isolation of the gene and hypothalamic of cDNA for the common precursor of gonadotropin-releasing hormone and prolactin release-inhibiting factor in human and rat

Abstract

Cloned cDNAs encoding the precursor protein for gonadotropin-releasing hormone (Gn-RH) and prolactin release-inhibiting factor (PIF) were isolated from libraries derived from human and rat hypothalamic mRNA. Nucleotide sequence analyses predict precursor proteins of 92 amino acids for both species and show identity between the human placental and human hypothalamic precursor proteins. Whereas the Gn-RH peptide structure is completely conserved in human and rat, the PIF domain of the precursor displays 70% interspecies homology. Genomic analyses revealed the presence of a single Gn-RH-PIF gene in human and rat containing sequences corresponding to the cDNA distributed across four exons.

Authors:
; ; ;
Publication Date:
Research Org.:
Genentech, Inc., San Francisco, CA
OSTI Identifier:
6155850
Resource Type:
Journal Article
Journal Name:
Proc. Natl. Acad. Sci. U.S.A.; (United States)
Additional Journal Information:
Journal Volume: 83:1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; LIBERINS; BIOCHEMISTRY; MESSENGER-RNA; DNA-CLONING; DNA SEQUENCING; ELECTROPHORESIS; GENETIC ENGINEERING; GONADOTROPINS; HYPOTHALAMUS; LH-RH; MAN; PHOSPHORUS 32; RATS; RECOMBINANT DNA; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; BRAIN; CENTRAL NERVOUS SYSTEM; CHEMISTRY; CLONING; DAYS LIVING RADIOISOTOPES; DNA; HORMONES; ISOTOPES; LIGHT NUCLEI; MAMMALS; NERVOUS SYSTEM; NUCLEI; NUCLEIC ACIDS; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; PEPTIDE HORMONES; PHOSPHORUS ISOTOPES; PITUITARY HORMONES; PRIMATES; RADIOISOTOPES; RNA; RODENTS; STRUCTURAL CHEMICAL ANALYSIS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Adelman, J P, Mason, A J, Hayflick, J S, and Seeburg, P H. Isolation of the gene and hypothalamic of cDNA for the common precursor of gonadotropin-releasing hormone and prolactin release-inhibiting factor in human and rat. United States: N. p., 1986. Web. doi:10.1073/pnas.83.1.179.
Adelman, J P, Mason, A J, Hayflick, J S, & Seeburg, P H. Isolation of the gene and hypothalamic of cDNA for the common precursor of gonadotropin-releasing hormone and prolactin release-inhibiting factor in human and rat. United States. https://doi.org/10.1073/pnas.83.1.179
Adelman, J P, Mason, A J, Hayflick, J S, and Seeburg, P H. 1986. "Isolation of the gene and hypothalamic of cDNA for the common precursor of gonadotropin-releasing hormone and prolactin release-inhibiting factor in human and rat". United States. https://doi.org/10.1073/pnas.83.1.179.
@article{osti_6155850,
title = {Isolation of the gene and hypothalamic of cDNA for the common precursor of gonadotropin-releasing hormone and prolactin release-inhibiting factor in human and rat},
author = {Adelman, J P and Mason, A J and Hayflick, J S and Seeburg, P H},
abstractNote = {Cloned cDNAs encoding the precursor protein for gonadotropin-releasing hormone (Gn-RH) and prolactin release-inhibiting factor (PIF) were isolated from libraries derived from human and rat hypothalamic mRNA. Nucleotide sequence analyses predict precursor proteins of 92 amino acids for both species and show identity between the human placental and human hypothalamic precursor proteins. Whereas the Gn-RH peptide structure is completely conserved in human and rat, the PIF domain of the precursor displays 70% interspecies homology. Genomic analyses revealed the presence of a single Gn-RH-PIF gene in human and rat containing sequences corresponding to the cDNA distributed across four exons.},
doi = {10.1073/pnas.83.1.179},
url = {https://www.osti.gov/biblio/6155850}, journal = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number = ,
volume = 83:1,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1986},
month = {Wed Jan 01 00:00:00 EST 1986}
}