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Title: Ras p21 and other Gn proteins are detected in mammalian cell lines by (gamma-/sup 35/S)GTP gamma S binding

Abstract

The presence of guanine nucleotide binding proteins in mouse and human cell lines was investigated using (gamma-/sup 35/S)GTP gamma S and (gamma-32P)GTP. Cell lysate polypeptides were separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis and transferred to nitrocellulose. Incubation of the nitrocellulose blots with (gamma-/sup 35/S)GTP gamma S identified 9 distinct GTP-binding polypeptides in all lysates. One of these is the ras oncogene product, p21, as demonstrated by subsequent immunochemical staining of the nitrocellulose blots. We have shown that this procedure provides a sensitive method for detection of p21 in culture cell lines.

Authors:
; ; ;
Publication Date:
Research Org.:
Univ. of East Anglia, Norwich (England)
OSTI Identifier:
6155768
Resource Type:
Journal Article
Journal Name:
Biochem. Biophys. Res. Commun.; (United States)
Additional Journal Information:
Journal Volume: 159:3
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; POLYPEPTIDES; BIOCHEMICAL REACTION KINETICS; ELECTROPHORESIS; ISOTOPE DILUTION; MAN; MEMBRANE PROTEINS; MICE; MOLECULAR WEIGHT; NUCLEOTIDES; PHOSPHORUS 32; SULFUR 35; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; EVEN-ODD NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; LIGHT NUCLEI; MAMMALS; NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PEPTIDES; PHOSPHORUS ISOTOPES; PRIMATES; PROTEINS; RADIOISOTOPES; REACTION KINETICS; RODENTS; SULFUR ISOTOPES; TRACER TECHNIQUES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Comerford, J G, Gibson, J R, Dawson, A P, and Gibson, I. Ras p21 and other Gn proteins are detected in mammalian cell lines by (gamma-/sup 35/S)GTP gamma S binding. United States: N. p., 1989. Web. doi:10.1016/0006-291X(89)92247-X.
Comerford, J G, Gibson, J R, Dawson, A P, & Gibson, I. Ras p21 and other Gn proteins are detected in mammalian cell lines by (gamma-/sup 35/S)GTP gamma S binding. United States. https://doi.org/10.1016/0006-291X(89)92247-X
Comerford, J G, Gibson, J R, Dawson, A P, and Gibson, I. 1989. "Ras p21 and other Gn proteins are detected in mammalian cell lines by (gamma-/sup 35/S)GTP gamma S binding". United States. https://doi.org/10.1016/0006-291X(89)92247-X.
@article{osti_6155768,
title = {Ras p21 and other Gn proteins are detected in mammalian cell lines by (gamma-/sup 35/S)GTP gamma S binding},
author = {Comerford, J G and Gibson, J R and Dawson, A P and Gibson, I},
abstractNote = {The presence of guanine nucleotide binding proteins in mouse and human cell lines was investigated using (gamma-/sup 35/S)GTP gamma S and (gamma-32P)GTP. Cell lysate polypeptides were separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis and transferred to nitrocellulose. Incubation of the nitrocellulose blots with (gamma-/sup 35/S)GTP gamma S identified 9 distinct GTP-binding polypeptides in all lysates. One of these is the ras oncogene product, p21, as demonstrated by subsequent immunochemical staining of the nitrocellulose blots. We have shown that this procedure provides a sensitive method for detection of p21 in culture cell lines.},
doi = {10.1016/0006-291X(89)92247-X},
url = {https://www.osti.gov/biblio/6155768}, journal = {Biochem. Biophys. Res. Commun.; (United States)},
number = ,
volume = 159:3,
place = {United States},
year = {Fri Mar 31 00:00:00 EST 1989},
month = {Fri Mar 31 00:00:00 EST 1989}
}