beta. -adrenergic receptor binding characteristics and responsiveness in cultured Wistar-Kyoto rat arterial smooth muscle cells

Journal Article · Fri Jan 01 00:00:00 EST 1988 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(88)90144-0· OSTI ID:6152566

Jazayeri, A; Meyer, III, W J

The tone of arterial blood vessels is regulated by the catecholamines through their receptors on arterial smooth muscle cells (ASMC). ..beta..-/sub 2/-adrenergic receptors of ASMC mediate vasodilation through agonist mediated c-AMP production. Previous reports have described these receptors on freshly isolated blood vessels. This study demonstrates the presence of ..beta../sub 2/-adrenergic receptors on cultured rat ASMC and that these receptors are functional. ..beta..-adrenergic receptor binding was measured using (/sup 3/H)-dihydroalprenolol (DHA) binding to the membrane of cultured ASMC from normotensive Wistar-Kyoto rats. The ASMC ..beta..-adrenergic receptors have a Kd of 0.56 +/- 0.16 nM and a Bmax of 57.2 +/- 21.7 fmol/mg protein. Competition binding studies revealed a much greater affinity of these receptors for epinephrine than norepinephrine, indicating the preponderance of a ..beta../sub 2/-adrenergic receptor subtype. Isoproterenol stimulation of cultured ASMC resulted in a 14 +/- 7 fold increase in intracellular c-AMP content of these cells indicating these receptors are functional. ..beta..-adrenergic receptors of cultured ASMC provide an excellent system in which the association between hypertension and observed ..beta..-adrenergic receptor differences can be further explored.

• Research Organization: Univ. of Texas Medical Branch, Galveston (USA)
• OSTI ID: 6152566
• Journal Information: Life Sci.; (United States), Vol. 43:8
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ARTERIES
VASODILATION
RECEPTORS
BIOCHEMICAL REACTION KINETICS
CHEMICAL PROPERTIES
SYMPATHOMIMETICS
AMP
CELL CULTURES
CELL MEMBRANES
HYPERTENSION
MUSCLES
RATS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BLOOD VESSELS
BODY
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
DISEASES
DRUGS
KINETICS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PROTEINS
REACTION KINETICS
RODENTS
SYMPTOMS
VASCULAR DISEASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques