Cytoplasmic dynein is a minus end-directed motor for membranous organelles

Journal Article · Fri Mar 24 00:00:00 EST 1989 · Cell; (United States)

DOI:https://doi.org/10.1016/0092-8674(89)90627-2· OSTI ID:6149021

Schroer, T A; Steuer, E R; Sheetz, M P

The role of cytoplasmic dynein in microtubule-based organelle transport was examined using a reconstituted assay developed from chick embryo fibroblasts. Factors present in a high-speed cytosol caused the movement of purified organelles on microtubules predominantly in the minus end direction. Inactivation of cytoplasmic dynein in the high-speed cytosol by vanadate-mediated UV photocleavage inhibited minus end-directed organelle motility by over 90%. Addition of purified cytoplasmic dynein to the inactive cytosol restored minus end-directed organelle motility, although purified cytoplasmic dynein by itself did not support organelle movement. We propose that cytoplasmic dynein is the motor for minus end-directed organelle movement, but that additional cytosolic factors are also required to produce organelle motility.

• Research Organization: Washington Univ. School of Medicine, St. Louis, MO (USA)
• OSTI ID: 6149021
• Journal Information: Cell; (United States), Vol. 56:6
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ORGANOIDS
MEMBRANE TRANSPORT
PROTEINS
BIOLOGICAL FUNCTIONS
BIOASSAY
BIOLOGICAL RADIATION EFFECTS
BRAIN
CELL MEMBRANES
CHICKENS
CYTOPLASM
EMBRYOS
FIBROBLASTS
MICROTUBULES
MOLECULAR WEIGHT
ULTRAVIOLET RADIATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIRDS
BODY
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
FOWL
FUNCTIONS
MEMBRANES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
RADIATION EFFECTS
RADIATIONS
SOMATIC CELLS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture