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Title: Nuclear magnetic resonance studies of intracellular ions in perfused from heart

Abstract

Intracellular sodium, potassium, and lithium were observed in a perfused frog heart by nuclear magnetic resonance (NMR) spectroscopy. A perfusate buffer containing the shift reagent, dysprosium tripolyphosphate, was used in combination with mathematical filtering or presaturation of the extracellular resonance to separate the intra- and extracellular sodium NMR signals. Addition of 10 ..mu..M ouabain to the perfusate, perfusion with a zero potassium, low-calcium buffer, and replacement of 66% of the perfusate sodium with lithium resulted in changes in the intracellular sodium levels. An increase of 45% in the intracellular sodium was observed when changing the pacing rate from 0 to 60 beats/min (with proportional changes for intermediate pacing rates). The ratio of intracellular potassium to sodium concentration was determined to be 2.3 by NMR, indicating that a substantial amount of the intracellular potassium is undetectable with these NMR method. In addition, intracellular lithium was observed during perfusion with a lithium-containing perfusate.

Authors:
;
Publication Date:
Research Org.:
Harvard Univ., Cambridge, MA (USA)
OSTI Identifier:
6148125
Resource Type:
Journal Article
Journal Name:
Am. J. Physiol.: Heart Circ. Physiol.; (United States)
Additional Journal Information:
Journal Volume: 21:6
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; HEART; NUCLEAR MAGNETIC RESONANCE; PERFUSED TISSUES; PHYSIOLOGY; DYSPROSIUM COMPOUNDS; FOURIER TRANSFORMATION; FROGS; IONS; LITHIUM 7; LITHIUM COMPOUNDS; OUABAIN; POTASSIUM 39; POTASSIUM COMPOUNDS; SODIUM 23; SODIUM COMPOUNDS; ALKALI METAL COMPOUNDS; ALKALI METAL ISOTOPES; AMPHIBIANS; ANIMAL TISSUES; ANIMALS; AQUATIC ORGANISMS; BODY; CARBOHYDRATES; CARDIAC GLYCOSIDES; CARDIOTONICS; CARDIOVASCULAR AGENTS; CARDIOVASCULAR SYSTEM; CHARGED PARTICLES; DRUGS; GLYCOSIDES; INTEGRAL TRANSFORMATIONS; ISOTOPES; LIGHT NUCLEI; LITHIUM ISOTOPES; MAGNETIC RESONANCE; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; POTASSIUM ISOTOPES; RARE EARTH COMPOUNDS; RESONANCE; SODIUM ISOTOPES; STABLE ISOTOPES; STROPHANTHINS; TISSUES; TRANSFORMATIONS; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Burnstein, D, and Fossel, E T. Nuclear magnetic resonance studies of intracellular ions in perfused from heart. United States: N. p., 1987. Web.
Burnstein, D, & Fossel, E T. Nuclear magnetic resonance studies of intracellular ions in perfused from heart. United States.
Burnstein, D, and Fossel, E T. 1987. "Nuclear magnetic resonance studies of intracellular ions in perfused from heart". United States.
@article{osti_6148125,
title = {Nuclear magnetic resonance studies of intracellular ions in perfused from heart},
author = {Burnstein, D and Fossel, E T},
abstractNote = {Intracellular sodium, potassium, and lithium were observed in a perfused frog heart by nuclear magnetic resonance (NMR) spectroscopy. A perfusate buffer containing the shift reagent, dysprosium tripolyphosphate, was used in combination with mathematical filtering or presaturation of the extracellular resonance to separate the intra- and extracellular sodium NMR signals. Addition of 10 ..mu..M ouabain to the perfusate, perfusion with a zero potassium, low-calcium buffer, and replacement of 66% of the perfusate sodium with lithium resulted in changes in the intracellular sodium levels. An increase of 45% in the intracellular sodium was observed when changing the pacing rate from 0 to 60 beats/min (with proportional changes for intermediate pacing rates). The ratio of intracellular potassium to sodium concentration was determined to be 2.3 by NMR, indicating that a substantial amount of the intracellular potassium is undetectable with these NMR method. In addition, intracellular lithium was observed during perfusion with a lithium-containing perfusate.},
doi = {},
url = {https://www.osti.gov/biblio/6148125}, journal = {Am. J. Physiol.: Heart Circ. Physiol.; (United States)},
number = ,
volume = 21:6,
place = {United States},
year = {Mon Jun 01 00:00:00 EDT 1987},
month = {Mon Jun 01 00:00:00 EDT 1987}
}