Immortalization of human foreskin keratinocytes by various human papillomavirus DNAs corresponds to their association with cervical carcinoma
Normal human foreskin keratinocytes cotransfected with the neomycin resistance gene and recombinant human papillomavirus (HPV) DNAs (types 16, 18, 31, and 33) that have a high or moderate association with cervical malignancy acquired immortality and contained integrated and transcriptionally active viral genomes. Only transcripts from the intact E6 and E7 genes were detected in at least one cell line, suggesting that one or both of these genes are responsible for immortalization. Recombinant HPV DNAs with low or no oncogenic potential for cervical cancer (HPV1a, -5, -6b, and -11) induced small G418-resistant colonies that senesced as did the nontransfected cells. These colonies contained only episomal virus DNA; therefore, integration of HPV sequences is important for immortalization of keratinocytes. This study suggests that the virus-encoded immortalization function contributes to the pathogenesis of cervical carcinoma.
- Research Organization:
- National Cancer Institute, Bethesda, MD (USA)
- OSTI ID:
- 6146244
- Journal Information:
- J. Virol.; (United States), Vol. 63:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARCINOMAS
PATHOGENESIS
DNA
AUTORADIOGRAPHY
SOMATIC CELLS
CELL TRANSFORMATIONS
VIRUSES
GENE RECOMBINATION
ANTIBIOTICS
GENES
KERATIN
PLASMIDS
SKIN
TRANSCRIPTION
UTERUS
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
BODY
CELL CONSTITUENTS
DISEASES
DRUGS
FEMALE GENITALS
MICROORGANISMS
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PARASITES
PROTEINS
SCLEROPROTEINS
550701* - Microbiology- Tracer Techniques