Human macrophage scavenger receptors: Primary structure, expression, and localization in atherosclerotic lesions

Journal Article · Sat Dec 01 00:00:00 EST 1990 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.87.23.9133· OSTI ID:6144540

Matsumoto, Akiyo; Itakura, Hiroshige; Kodama, Tatsuhiko ^[1]; Naito, Makoto; Takahashi, Kiyoshi ^[2]; Ikemoto, Shinji; Asaoka, Hitoshi; Hayakawa, Ikuho ^[3]; Kanamori, Hiroshi; Takaku, Fumimaro ^[4]; Aburatani, Hiroyuki ^[5]; Suzuki, Hiroshi; Kobari, Yukage; Miyai, Tatsuya ^[6]; Cohen, E H; Wydro, R ^[7]; Housman, D E ^[8]

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1. Univ. of Tokyo (Japan) National Inst. of Health and Nutrition, Tokyo (Japan)
2. Kumamoto Univ. (Japan)
3. National Inst. of Health and Nutrition, Tokyo (Japan)
4. Univ. of Tokyo (Japan)
5. Univ. of Tokyo (Japan) Massachusetts Inst. of Tech., Cambridge, MA (United States)
6. Chugai Pharmaceutical, Tokyo (Japan)
7. Genzyme Corp., Framingham, MA (United States)
8. Massachusetts Inst. of Tech., Cambridge (United States)

Two types of cDNAs for human macrophage scavenger receptors were cloned from a cDNA library derived from the phorbol ester-treated human monocytic cell line THP-1. The type I and type II human scavenger receptors encoded by these cDNAs are homologous (73% and 71% amino acid identity) to their previously characterized bovine counterparts and consist of six domains: cytoplasmic (I), membrane-spanning (II), spacer (III), {alpha}-helical coiled-coil (IV), collagen-like (V), and a type-specific C-terminal (VI). The receptor gene is located on human chromosome 8. The human receptors expressed in CHO-K1 cells mediated endocytosis of modified low density lipoproteins. Two mRNAs, 4.0 and 3.2 kilobases, have been detected in human liver, placenta, and brain. Immunohistochemical studies using an anti-peptide antibody which recognizes human scavenger receptors indicated the presence of the scavenger receptors in the macrophages of lipid-rich atherosclerotic lesions, suggesting the involvement of scavenger receptors in atherogenesis.

• OSTI ID: 6144540
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 87:23; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ARTERIOSCLEROSIS
PATHOGENESIS
RECEPTORS
AMINO ACID SEQUENCE
RECOMBINANT DNA
DNA SEQUENCING
BRAIN
CYTOCHEMISTRY
DNA-CLONING
HETEROCHROMOSOMES
LIVER
MACROPHAGES
MESSENGER-RNA
PHORBOL ESTERS
PHOSPHORUS 32
PLACENTA
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BODY
CARCINOGENS
CARDIOVASCULAR DISEASES
CENTRAL NERVOUS SYSTEM
CHEMISTRY
CHROMOSOMES
CLONING
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
DNA
DNA HYBRIDIZATION
ESTERS
FETAL MEMBRANES
GLANDS
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MEMBRANE PROTEINS
MEMBRANES
MOLECULAR STRUCTURE
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHAGOCYTES
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RNA
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
VASCULAR DISEASES
550201* - Biochemistry- Tracer Techniques