Qualitative analysis of mycotoxins using micellar electrokinetic capillary chromatography
- Univ. of Tennessee, Knoxville (United States)
Naturally occurring mycotoxins are separated using micellar electrokinetic capillary chromatography. Trends in the retention of these toxins, resulting from changes in mobile-phase composition and pH, are reported and presented as a means of alleviating coelution problems. Two sets of mobile-phase conditions are determined that provide unique separation selectivity. The facile manner by which mobile-phase conditions can be altered, without changes in instrumental configuration, allowed the acquisition of two distinctive, fully resolved chromatograms of 10 mycotoxins in a period of approximately 45 min. By adjusting retention times, using indigenous or added components in mycotoxin samples as normalization standards, it is possible to obtain coefficients of variation in retention time that average less than 1%. The qualitative capabilities of this methodology are evaluated by separating randomly generated mycotoxin-interferent mixtures. In this study, the utilization of normalized retention times applied to separations obtained with two sets of mobile-phase conditions permitted the identification of all the mycotoxins in five unknown samples without any misidentifications. 24 refs., 3 figs., 2 tabs.
- DOE Contract Number:
- FG05-86ER13613
- OSTI ID:
- 6139265
- Journal Information:
- Analytical Chemistry (Washington); (United States), Vol. 65:9; ISSN 0003-2700
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
TOXINS
QUALITATIVE CHEMICAL ANALYSIS
CAPILLARIES
CHROMATOGRAPHY
ELECTRODYNAMICS
EXPERIMENTAL DATA
FUNGI
METABOLITES
MICELLAR SYSTEMS
ANTIGENS
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
CHEMICAL ANALYSIS
DATA
HAZARDOUS MATERIALS
INFORMATION
MATERIALS
NUMERICAL DATA
ORGANS
PLANTS
SEPARATION PROCESSES
TOXIC MATERIALS
560300* - Chemicals Metabolism & Toxicology
400100 - Analytical & Separations Chemistry