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Title: Contact microscopy with synchrotron radiation

Soft x-ray contact microscopy with synchrotron radiation offers the biologist and especially the microscopist, a way to morphologically study specimens that could not be imaged by conventional TEM, STEM or SEM methods (i.e. hydrated samples, samples easily damaged by an electron beam, electron dense samples, thick specimens, unstained low contrast specimens) at spatial resolutions approaching those of the TEM, with the additional possibility to obtain compositional (elemental) information about the sample as well. Although flash x-ray sources offer faster exposure times, synchrotron radiation provides a highly collimated, intense radiation that can be tuned to select specific discrete ranges of x-ray wavelengths or specific individual wavelengths which optimize imaging or microanalysis of a specific sample. This paper presents an overview of the applications of x-ray contact microscopy to biological research and some current research results using monochromatic synchrotron radiation to image biological samples. 24 refs., 10 figs.
Authors:
Publication Date:
OSTI Identifier:
6138393
Report Number(s):
BNL-37328; CONF-8505112-5
ON: DE86005266
DOE Contract Number:
AC02-76CH00016
Resource Type:
Conference
Resource Relation:
Conference: 2. international symposium on the biology and toxicology of metals using nuclear analytical methods, Upton, NY, USA, 20 May 1985; Other Information: Portions of this document are illegible in microfiche products
Research Org:
State Univ. of New York, Stony Brook (USA). Dept of Allied Health Resources and Anatomical Sciences; Brookhaven National Lab., Upton, NY (USA)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION; MICROSCOPY; PERFORMANCE TESTING; SYNCHROTRON RADIATION; USES; DESIGN; MICRORADIOGRAPHY; MUCOPOLYSACCHARIDES; SOFT X RADIATION; AMINES; BREMSSTRAHLUNG; CARBOHYDRATES; ELECTROMAGNETIC RADIATION; IONIZING RADIATIONS; ORGANIC COMPOUNDS; POLYSACCHARIDES; RADIATIONS; SACCHARIDES; TESTING; X RADIATION 550300* -- Cytology; 440300 -- Miscellaneous Instruments-- (-1989)