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Title: Rapid radiometric methods to detect and differentiate Mycobacterium tuberculosis/M. bovis from other mycobacterial species

Rapid methods for the differentiation of Mycobacterium tuberculosis/M. bovis (TB complex) from other mycobacteria (MOTT bacilli) were developed and evaluated in a three-phase study. In the first phase, techniques for identification of Mycobacterium species were developed by using radiometric technology and BACTEC Middlebrook 7H12 liquid medium. Based on /sup 14/CO/sub 2/ evolution, characteristic growth patterns were established for 13 commonly encountered mycobacterial species. Mycobacteria belonging to the TB complex were differentiated from other mycobacteria by cellular morphology and rate of /sup 14/CO/sub 2/ evolution. For further differentiation, radiometric tests for niacin production and inhibition by Q-nitro-alpha-acetyl amino-beta-hydroxy-propiophenone (NAP) were developed. In the second phase, 100 coded specimens on Lowenstein-Jensen medium were identified as members of the TB complex, MOTT bacilli, bacteria other than mycobacteria, or ''no viable organisms'' within 3 to 12 (average 6.4) days of receipt from the Centers for Disease Control. Isolation and identification of mycobacteria from 20 simulated sputum specimens were carried out in phase III. Out of 20 sputum specimens, 16 contained culturable mycobacteria, and all of the positives were detected by the BACTEC method in an average of 7.3 days. The positive mycobacterial cultures were isolated and identified as TB complex or MOTT bacilli inmore » an average of 12.8 days. The radiometric NAP test was found to be highly sensitive and specific for a rapid identification of TB complex, whereas the radiometric niacin test was found to have some inherent problems. Radiometric BACTEC and conventional methodologies were in complete agreement in Phase II as well as in Phase III.« less
Authors:
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Publication Date:
OSTI Identifier:
6136194
Resource Type:
Journal Article
Resource Relation:
Journal Name: Am. Rev. Respir. Dis.; (United States); Journal Volume: 130:4
Research Org:
Johnston Labs., Towson, MD
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; MYCOBACTERIUM; RADIOMETRIC ANALYSIS; TAXONOMY; TUBERCULOSIS; DIAGNOSIS; CARBON 14 COMPOUNDS; CARBON DIOXIDE; CELL CULTURES; MYCOBACTERIUM TUBERCULOSIS; SALIVA; SPECIES DIVERSITY; TRACER TECHNIQUES; BACTERIA; BACTERIAL DISEASES; BIOLOGICAL MATERIALS; BIOLOGY; BODY FLUIDS; CARBON COMPOUNDS; CARBON OXIDES; CHALCOGENIDES; CHEMICAL ANALYSIS; DISEASES; INFECTIOUS DISEASES; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MATERIALS; MICROORGANISMS; OXIDES; OXYGEN COMPOUNDS; QUANTITATIVE CHEMICAL ANALYSIS 550701* -- Microbiology-- Tracer Techniques