Biosynthesis and transport of gangliosides in peripheral nerve

Journal Article · Sun Jan 01 00:00:00 EST 1984 · Adv. Exp. Med. Biol.; (United States)

DOI:https://doi.org/10.1007/978-1-4684-1200-0_14· OSTI ID:6133475

Yates, A J; Tipnis, U R; Hofteig, J H; Warner, J K

Radiolabelled glucosamine was injected into L-7 dorsal root ganglion (DRG) of rabbits. At several different times after injection DRG, lumbosacral trunks (LST) and sciatic nerves (SN) were removed and gangliosides extracted. Two and 3 weeks after injection the amounts of radioactivity in the ganglioside fractions of LST and SN were significantly higher than at days 1 and 2. The TCA soluble radioactivity decreased dramatically over the same time period. Colchicine prevented the appearance of radiolabelled lipid in LST and SN. From these experiments the authors conclude that some ganglioside is synthesized in the neuronal cell bodies of DRG and transported in the axons of the sciatic nerve. In another experiment the sciatic nerve was transected and ends separated to prevent regeneration. There was no difference in the amount of radiolabelled ganglioside that was isolated from DRG or LST of transected nerves compared with control nerves. The behavior of several potential acid soluble contaminants was studied in several steps used to isolate gangliosides. Of those studied only CMP-NeuAc could cause significant contamination of the final ganglioside preparation.

• Research Organization: Ohio State Univ., Columbus
• OSTI ID: 6133475
• Journal Information: Adv. Exp. Med. Biol.; (United States), Vol. 174
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GANGLIOSIDES
BIOSYNTHESIS
MEMBRANE TRANSPORT
TRITIUM COMPOUNDS
GANGLIONS
GLUCOSAMINE
NERVES
RABBITS
AMINES
ANIMALS
CARBOHYDRATES
HEXOSAMINES
HEXOSES
LABELLED COMPOUNDS
LIPIDS
MAMMALS
MONOSACCHARIDES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
SACCHARIDES
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques