Enzymology of the P-C bond. A study of the biosynthesis and biodegradation of 2-aminoethylphosphonate
The mechanisms by which phosphorus-carbon bonds are made and are broken in biological systems are not well understood. In their laboratory they have examined the biosynthesis and biodegradation of the most predominate phosphonate in nature, 2-aminoethylphosphonate (AEP). In Bacillus cereus AEP is first converted to phosphonoacetaldehyde (PA) before hydrolysis of the P-C linkage takes place. They have examined the catalytic mechanism of the enzyme which catalyzes this hydrolysis, phosphonoacetaldehyde hydrolase (phosphonatase). Treatment of the enzyme with (/sup 3/H)-PA and NaBH/sub 4/ resulted in ethylation of an active site lysine and loss of catalytic activity. Thus, hydrolysis appears to occur via a covalent imine intermediate. Their studies of AEP biosynthesis in Tetrahymena pyriformis have focused on testing the precursor roles of PEP, phosphonopyruvate and PA. Resuspended microsomes were found to catalyze in the presence of pyridoxal phosphate and alanine, the conversion of these three precursors to AEP. The P-C bond appears to be formed by rearrangement of PEP.
- Research Organization:
- Univ. of Maryland, College Park
- OSTI ID:
- 6126096
- Report Number(s):
- CONF-870644-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
- Country of Publication:
- United States
- Language:
- English
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Investigation into the mechanism of the Bacillus cereus phosphonoacetaldehyde hydrolase enzyme
Related Subjects
HYDROLASES
ENZYME ACTIVITY
PHOSPHONATES
BIOSYNTHESIS
METABOLISM
BACILLUS CEREUS
HYDROLYSIS
MICROSOMES
PRECURSOR
TETRAHYMENA
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
BACILLUS
BACTERIA
CELL CONSTITUENTS
CHEMICAL REACTIONS
CILIATA
DECOMPOSITION
ENZYMES
INVERTEBRATES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LYSIS
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
PROTOZOA
SOLVOLYSIS
SYNTHESIS
550201* - Biochemistry- Tracer Techniques