ATP-dependent degradation of /sup 125/I-bovine serum albumin by rabbit reticulocytes does not need repression of an endogenous inhibitor
The ubiquitin-dependent proteolysis of /sup 125/I-bovine serum albumin in rabbit reticulocytes has been investigated. Using various reticulocyte fractions (reticulocyte protease, inhibitor-free protease, ubiquitin and inhibitor) in the presence or absence of ATP, we found that the repression of an endogenous inhibitor, as suggested by others for alpha-casein proteolysis, is unlikely for bovine serum albumin. Therefore, differences exist in the ATP-dependent proteolytic pathway of rabbit reticulocytes depending on the substrate. Fractionation of the reticulocyte ATP-dependent proteolytic system revealed at least two proteolytic and two inhibitory fractions involved in the proteolysis of bovine serum albumin.
- Research Organization:
- Instituto de Investigaciones Citologicas de la Caja de Ahorros de Valencia, Spain
- OSTI ID:
- 6125456
- Journal Information:
- Physiol. Chem. Phys. Med. NMR; (United States), Vol. 19:1
- Country of Publication:
- United States
- Language:
- English
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ALBUMINS
PROTEOLYSIS
ATP
BIOLOGICAL PATHWAYS
CATTLE
ENZYME INHIBITORS
FRACTIONATION
IODINE 125
PEPTIDE HYDROLASES
RABBITS
RETICULOCYTES
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
ERYTHROCYTES
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
NUCLEI
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
RUMINANTS
SEPARATION PROCESSES
VERTEBRATES
550501* - Metabolism- Tracer Techniques