Biochemical studies on strain differences of mice in the susceptibility to nitrogen dioxide

Journal Article · Mon Nov 01 00:00:00 EST 1982 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(82)90035-2· OSTI ID:6117818

Ichinose, T; Suzuki, A K; Tsubone, H; Sagai, M

Strain differences of mice in their susceptibility to nitrogen dioxide (NO/sub 2/) were examined by measuring the activities of antioxidative protective enzymes, and the amounts of antioxidants and lipid peroxides in lungs. Four strains of mice: ICR, BALB/c, ddy and C57BL/6 were used in this study and their LC/sub 50/ values after exposure to NO/sub 2/ for 16 hr were: 38, 49, 51 and 64 ppm, respectively. Genetic strain differences were observed in the enzyme activities, the antioxidant contents and lipid peroxide contents among these four different strains. The activities of glutathione peroxidase (GP/sub x/), glutathione S-transferase, and superoxide dismutase (SOD), and the contents of non-protein sulfyhdryls (NPSH), ..cap alpha..-tocopherol (..cap alpha..-Toc) and total lipids in lungs of the four strains were related to their LC/sub 50/, while TBA reactants in lungs of the four strains were inversely related to their LC/sub 50/. After exposure to 20 ppm NO/sub 2/ for 16 hr, the activities of the protective enzymes and the contents of NPSH decreased, while the level of ..cap alpha..-Toc increased markedly. The activities of GP/sub x/, 6-phosphogluconate dehydrogenase, SOD and disulfide reductase, and the contents of NPSH, ..cap alpha..-Toc and total lipids were also related to their LC/sub 50/. On the other hand, TBA reactants increased higher than those of the control groups and were inversely related to their LC/sub 50/. These results suggest that the protective enzymes and the antioxidants are important factors as defence mechanism in lungs to NO/sub 2/ and that the intensity of the protective systems in pigmented strains is generally greater than that in albino strains.

• OSTI ID: 6117818
• Journal Information: Life Sci.; (United States), Vol. 31:18
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
MICE
GENETIC VARIABILITY
NITROGEN DIOXIDE
BIOLOGICAL EFFECTS
ANTIOXIDANTS
DOSE-RESPONSE RELATIONSHIPS
ENZYME ACTIVITY
LIPIDS
LUNGS
PEROXIDES
RESPONSE MODIFYING FACTORS
ANIMALS
BIOLOGICAL VARIABILITY
BODY
CHALCOGENIDES
MAMMALS
NITROGEN COMPOUNDS
NITROGEN OXIDES
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
RESPIRATORY SYSTEM
RODENTS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)