Lack of induction of single-strand breaks in mammalian cells by sodium azide and its proximal mutagen
Abstract
The mutagenicity of sodium azide in both higher plants and bacteria is well documented. However, in mammalian cells, research on the effects of azide on gene mutations has produced conflicting results. Furthermore, no research has been conducted on the effects of azide and its proximal mutagen (mutagenic metabolite) on single-strand breaks. The experiments herein were designed to overcome this lack of information on azide mutagenicity and to evaluate the potential hazard of azide exposure to man. Chinese hamster V/sub 79/ cells were treated with either azide or its proximal mutagen(s) for 2 h or 6 h respectively and analyzed by alkaline elution for single-strand breaks. The data showed that there was no effect of either azide or the proximal mutagen(s) on single-strand DNA breaks nor was there any indication that azide or the proximal mutagen(s) induced DNA-protein crosslinks. The data suggest that neither azide nor its proximal mutagen(s) interact directly with DNA and suggest that the methods may be applied to any specified population and dose scenario.
- Authors:
- Publication Date:
- Research Org.:
- Washington State Univ., Pullman (USA). Coll. of Agriculture
- OSTI Identifier:
- 6111002
- Report Number(s):
- DOE/EV/72002-56
ON: DE82001721
- DOE Contract Number:
- AT06-76EV72002
- Resource Type:
- Technical Report
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ANIMAL CELLS; GENE MUTATIONS; AZIDES; MUTAGENESIS; DNA; CROSS-LINKING; STRAND BREAKS; CELL CULTURES; HAMSTERS; METABOLITES; PROTEINS; SODIUM COMPOUNDS; ALKALI METAL COMPOUNDS; ANIMALS; CHEMICAL REACTIONS; MAMMALS; MUTATIONS; NITROGEN COMPOUNDS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; POLYMERIZATION; RODENTS; VERTEBRATES; 560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
Citation Formats
Arenaz, P, Nilan, R A, and Kleinhofs, A. Lack of induction of single-strand breaks in mammalian cells by sodium azide and its proximal mutagen. United States: N. p., 1981.
Web.
Arenaz, P, Nilan, R A, & Kleinhofs, A. Lack of induction of single-strand breaks in mammalian cells by sodium azide and its proximal mutagen. United States.
Arenaz, P, Nilan, R A, and Kleinhofs, A. 1981.
"Lack of induction of single-strand breaks in mammalian cells by sodium azide and its proximal mutagen". United States.
@article{osti_6111002,
title = {Lack of induction of single-strand breaks in mammalian cells by sodium azide and its proximal mutagen},
author = {Arenaz, P and Nilan, R A and Kleinhofs, A},
abstractNote = {The mutagenicity of sodium azide in both higher plants and bacteria is well documented. However, in mammalian cells, research on the effects of azide on gene mutations has produced conflicting results. Furthermore, no research has been conducted on the effects of azide and its proximal mutagen (mutagenic metabolite) on single-strand breaks. The experiments herein were designed to overcome this lack of information on azide mutagenicity and to evaluate the potential hazard of azide exposure to man. Chinese hamster V/sub 79/ cells were treated with either azide or its proximal mutagen(s) for 2 h or 6 h respectively and analyzed by alkaline elution for single-strand breaks. The data showed that there was no effect of either azide or the proximal mutagen(s) on single-strand DNA breaks nor was there any indication that azide or the proximal mutagen(s) induced DNA-protein crosslinks. The data suggest that neither azide nor its proximal mutagen(s) interact directly with DNA and suggest that the methods may be applied to any specified population and dose scenario.},
doi = {},
url = {https://www.osti.gov/biblio/6111002},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1981},
month = {Thu Jan 01 00:00:00 EST 1981}
}