Studies on the interaction of lidocaine with plasma proteins
This study sought to quantitate lidocaine's interaction with alpha-1-acid glycoprotein (AAG), human serum albumin (HSA), and AAG in the presence of HSA, and to determine the extent of displacement of lidocaine from its binding site(s) by selected cardiovascular drugs (dipyridamole, disopyramide and quinidine). Since the limited experimental work reported in this area has involved the use of a single lidocaine concentration, this study involved the evaluation of a range of lidocaine concentrations. Lidocaine interaction with plasma proteins (AAG and HSA) was studied at 37/sup 0/C using an isothermal equilibrium dialysis system and /sup 14/C-lidocaine HCl. A dialysis membrane (M.W. cutoff 12,000 to 14,000) separated the two chambers of each dialysis cell. The extent of /sup 14/C-lidocaine dialysis was studied with respect to both drug and protein concentrations. Aliquots of each chamber of each of the cells were subjected to liquid scintillation counting (LSC) analyses for /sup 14/C-lidocaine. The ratio of bound to free (R/F) lidocaine was evaluated as a function of AAG concentration from the LSC data. Scatchard and/or Rosenthal analyses were employed to evaluate n and k values where appropriate. Linear and multiple linear regression analyses of the data were appropriately performed.
- Research Organization:
- Duquesne Univ., Pittsburgh, PA (USA)
- OSTI ID:
- 6108162
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
BIOCHEMICAL REACTION KINETICS
ANESTHETICS
CARDIOVASCULAR AGENTS
BIOLOGICAL EFFECTS
GLUCOPROTEINS
CARBON 14 COMPOUNDS
EXPERIMENTAL DATA
MEASURING METHODS
TRACER TECHNIQUES
CARBOHYDRATES
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DATA
DRUGS
INFORMATION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
NUMERICAL DATA
ORGANIC COMPOUNDS
PROTEINS
REACTION KINETICS
SACCHARIDES
550600* - Medicine
550201 - Biochemistry- Tracer Techniques