Studies on the biology of the crisamicin-producing organism Micromonospora purpureochromogenes subsp. celinoensis
A Micromonospora isolate, RV-101, obtained from the Red V Coconut Company in the Philippines, produces a new complex of antibiotics, the crisamicins. Using standard taxonomic methods for the genus Micromonospora, including micromorphology, growth characteristics on select media, whole cell analysis of chemical composition, and carbohydrate utilization patterns, the organism was classified as Micromonospora purpureochromogenes subsp. celinoensis. The chief character used in this classification was the production of a dark-brown diffusible pigment on media containing complex sources of nitrogen. The biosynthesis of crisamicin A was investigated by the technique of /sup 13/C acetate feeding and /sup 13/C NMR spectroscopy. Crisamicin A was found to be synthesized from acetate via the polyketide biosynthetic pathway. In addition, the assignment of one of two possible structures, differing in the position of phenolic hydroxyl groups, and in the point of linkage between the two monomers of the molecule, was made using the labeling data. The structure determined demonstrates that crisamicin A is unique among the benzoisochromanequinone antibiotics, in that it lacks an oxygen atom at position C-8.
- Research Organization:
- Rutgers-the State Univ., New Brunswick, NJ (USA)
- OSTI ID:
- 6106725
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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ANTIBIOTICS
BIOSYNTHESIS
MOLECULAR STRUCTURE
BACTERIA
GROWTH
BIOLOGICAL PATHWAYS
CARBON 13
NUCLEAR MAGNETIC RESONANCE
TRACER TECHNIQUES
ANTI-INFECTIVE AGENTS
CARBON ISOTOPES
DRUGS
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MICROORGANISMS
NUCLEI
RESONANCE
STABLE ISOTOPES
SYNTHESIS
550701* - Microbiology- Tracer Techniques
550201 - Biochemistry- Tracer Techniques