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Title: Monoclonal antibodies specific for sickle cell hemoglobin

Two mouse hybridoma cell lines were isolated which produce monoclonal antibodies that bind hemoglobin S. The mice were immunized with peptide-protein conjugates to stimulate a response to the amino terminal peptide of the beta chain of hemoglobin S, where the single amino acid difference between A and S occurs. Immunocharacterization of the antibodies shows that they bind specifically to the immunogen peptide and to hemoglobin S. The specificity for S is high enough that one AS cell in a mixture with a million AA cells is labeled by antibody, and such cells can be analyzed by flow cytometry. Immunoblotting of electrophoretic gels allows definitive identification of hemoglobin S as compared with other hemoglobins with similar electrophoretic mobility. 12 references, 4 figures.
Authors:
; ; ; ; ;
Publication Date:
OSTI Identifier:
6105446
DOE Contract Number:
W-7405-ENG-48
Resource Type:
Journal Article
Resource Relation:
Journal Name: Hemoglobin; (United States); Journal Volume: 9:4
Research Org:
Lawerence Livermore National Lab., CA
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; HEMOGLOBIN; IMMUNE REACTIONS; SCREENING; MONOCLONAL ANTIBODIES; BIOCHEMICAL REACTION KINETICS; AMINO ACIDS; CELL FLOW SYSTEMS; ELECTROPHORESIS; HYBRIDOMAS; MICE; PEPTIDES; SICKLE CELL ANEMIA; ANEMIAS; ANIMAL CELLS; ANIMALS; ANTIBODIES; CARBOXYLIC ACIDS; DISEASES; GLOBIN; HEMIC DISEASES; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; KINETICS; MAMMALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PIGMENTS; PORPHYRINS; PROTEINS; REACTION KINETICS; RODENTS; SYMPTOMS; VERTEBRATES 550200* -- Biochemistry