Autoradiographic localization of thyrotropin releasing hormone (TRH) receptors in the central nervous system

Thesis/Dissertation · Tue Jan 01 00:00:00 EST 1985

OSTI ID:6105228

Manaker, S

Quantitative autoradiography was used to examine the distribution of thyrotropin-releasing hormone (TRH) receptors in the rat and human central nervous system (CNS). The binding of (/sup 3/H)-3-methyl-histidine/sup 2/-TRH ((/sup 3/H)-MeTRH) to TRH receptors was saturable, of a high affinity (K/sub d/ = 5 nM), and specific for TRH analogs. Studies with neurotoxins ibotenic acid and 6-hydroxydopamine (6-OHDA) suggest that TRH receptors within the amygdala are predominantly located on cell bodies, and not nerve terminals. Finally, an examination was made of the concentrations of TRH receptors in spinal cords of patients with amyotrophic lateral sclerosis (ALS), a degenerative disease of the motor neurons located in Lamina IX. Large decreases in TRH receptors were noted in ALS spinal cords, when compared to non-neurological controls, probably reflecting the loss of motor neurons. In addition, decreases in the TRH receptor concentration of Lamina II were observed. This finding may reflect the sensitivity of neurons throughout the CNS to the pathophysiologic mechanisms of neuronal degeneration which cause ALS.

• Research Organization: Pennsylvania Univ., Philadelphia (USA)
• OSTI ID: 6105228
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
NERVOUS SYSTEM DISEASES
PATHOLOGY
PITUITARY HORMONES
BIOCHEMICAL REACTION KINETICS
RECEPTORS
AUTORADIOGRAPHY
CENTRAL NERVOUS SYSTEM
EXPERIMENTAL DATA
MAN
PATIENTS
RATS
TRACER TECHNIQUES
ANIMALS
DATA
DISEASES
HORMONES
INFORMATION
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
NUMERICAL DATA
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PRIMATES
PROTEINS
REACTION KINETICS
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
550901 - Pathology- Tracer Techniques