Studies on the biosynthesis and intracellular transport of gangliosides

Thesis/Dissertation · Thu Jan 01 00:00:00 EST 1987

OSTI ID:6101362

Farrer, R G

Ganglioside biosynthesis and transport to myelin was studied in brainstem of 17-21 day old rats. Brainstem slices were incubated for up to 2 hours with (/sup 3/H)glucosamine, and gangliosides were isolated by column chromatography and HPTLC. Results from these experiments showed that: (a) ganglioside synthesis was decreased in the slices compared to in vivo, and this decrease was greater in the more complex gangliosides than in the simpler ones; (b) label incorporation into gangliosides GM3 and GM2 increased in a linear fashion, whereas the rate of incorporation continuously increased over the 2 hour period for the more complex gangliosides; (c) label incorporated into gangliosides, which showed almost no effect of chase after 30 minutes; (d) monensin at 0.1 uM inhibited the synthesis of all gangliosides except GM3, GM2 and GD3. Compartmentation of ganglioside biosynthesis was examined by analyzing the subcellular location of two ganglioside synthesizing enzymes, lactosylceramide sialosyltransferase (LCST) and GDlb sialosyltransferase (GDlbST), acting early and late in the ganglioside pathway, respectively.

• Research Organization: Wayne State Univ., Detroit, MI (USA)
• OSTI ID: 6101362
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GANGLIOSIDES
MEMBRANE TRANSPORT
TRANSFERASES
SUBCELLULAR DISTRIBUTION
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
BRAIN
ENZYME ACTIVITY
GLUCOSAMINE
LIQUID COLUMN CHROMATOGRAPHY
RATS
TRACER TECHNIQUES
AMINES
ANIMALS
BODY
CARBOHYDRATES
CENTRAL NERVOUS SYSTEM
CHROMATOGRAPHY
DISTRIBUTION
ENZYMES
GLYCOLIPIDS
HEXOSAMINES
HEXOSES
ISOTOPE APPLICATIONS
LIPIDS
MAMMALS
MONOSACCHARIDES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
RODENTS
SACCHARIDES
SEPARATION PROCESSES
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques